Andrew Richards: Difference between revisions
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[[Andrew:Notebook/directTn5150303 | Direct Tn5 with 1-02 NeuN+ Ctx on C1 3/3/2015]] | [[Andrew:Notebook/directTn5150303 | Direct Tn5 with 1-02 NeuN+ Ctx on C1 3/3/2015]] | ||
[[Andrew:Notebook/C1_InTube_31nL_MDA_150320 | In tube validation for 31 nL MDA protocol 3/20/2015]] | |||
[[Andrew:Notebook/C1_InTube_23nL_MDA_150324 | In tube validation for 23 nL MDA protocol 3/24/2015]] | |||
[[Andrew:Notebook/C1_InTube_ALS_150330 | In tube validation for ALS protocol 3/30/2015]] | |||
[[Andrew:Notebook/C1_InTube_HLS_150333 | In tube validation for HLS protocol 3/33/2015]] | |||
===Direct Tn5=== | ===Direct Tn5=== |
Revision as of 20:32, 27 March 2015
Notebook
MagVigen DNA Select Focus 300/600 beads, October 1, 2014
Bulk Nextera libraries
January 25, 2015 (SW480 and lymphocyte)
February 10, 2015 (1-02 and 25-01)
MIDAS
C1 DNA Experiments
C1 DNA 5-10 um Chip on July 30, 2014
Direct Tn5 with 1-02 NeuN+ Ctx on C1 3/3/2015
In tube validation for 31 nL MDA protocol 3/20/2015
In tube validation for 23 nL MDA protocol 3/24/2015
In tube validation for ALS protocol 3/30/2015
In tube validation for HLS protocol 3/33/2015
Direct Tn5
Tn5059 (super mutant) test, February 17, 2015
In tube validation of Direct Tn5 C1 protocol
Protocols
Glass micropipette fabrication
MDA on microarray as of September 5, 2013
MDA amplicon extraction and processing
MDA amplicon extraction and processing as of September 5, 2013
MDA amplicon tagmentation and PCR
MDA amplicon tagmentation and PCR as of September 5, 2013
Technics PEIIB Planar Etcher for PDMS surface activation
Neuronal nuclei isolation as of December 3, 2013
Brandon's protocol for DNA purification after guanidine HCl
Computational stuff
Email from Jeff with tips on CNV calling
Sorts
2014 May 20 sort for 25-00 AD and 7-03 Control
2014 October 09 sort for 1-02 AD and 25-01 Control
2014 October 21 sort for SW480 and lymphocytes
Samples
Sequenced libraries for CNV analysis
- This is old. See Samples for full information
- This is also old. See Sequenced libraries
Reagents
High molecular weight (HMW) buffer recipe
- From Rui's wiki
Hypotonic lysis solution (HLS)
Nextera XT v2 primers (8 x 12)
Slides
Progress report for February 26, 2013
Progress report for February 26, 2013 REVISED
Progress report for April 25, 2013
Progress report for June 5, 2013
Progress report for September 04, 2013