Rui:RNAseq analysis on 121226 HL140: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>RuiLiu
(Created page with "==RNAseq on 121226_HL140== * Continue with Kun's note [http://genome-tech.ucsd.edu/LabNotes/index.php/Kun:LabNotes/SingleCellExpr/2013-1-1] * A number of questions that I hope...")
 
>RuiLiu
Line 24: Line 24:
|-
|-
| total Reads||||3,146,372||2,366,588||4,194,796||3,431,696
| total Reads||||3,146,372||2,366,588||4,194,796||3,431,696
|-
|}
| ||||||||||
 
|-
{| {{table}} border=1
| Input ng||#||N2.id05_avg||N2.id06_avg||N2.id07_avg||N2.id08_avg
| align="center" style="background:#f0f0f0;"|'''Input ng'''
| align="center" style="background:#f0f0f0;"|'''#'''
| align="center" style="background:#f0f0f0;"|'''N2.id05_avg'''
| align="center" style="background:#f0f0f0;"|'''N2.id06_avg'''
| align="center" style="background:#f0f0f0;"|'''N2.id07_avg'''
| align="center" style="background:#f0f0f0;"|'''N2.id08_avg'''
|-
|-
| 0||2||0.70%||0.84%||3.47%||3.53%
| 0||2||0.70%||0.84%||3.47%||3.53%

Revision as of 00:02, 3 January 2013

RNAseq on 121226_HL140

  • Continue with Kun's note [1]
  • A number of questions that I hope to answer with this data set include:
  1. Is there any indication that we can obtain reasonable data from 10pg of input total RNA?
  2. Is there a difference in efficiency between TSOs with and without the 8bp barcodes?
  3. Is there a difference in efficiency between T20V and T20VN primer?
  4. Is there a difference in efficiency between 72C and 10C annealing?
  5. Is NTC clean enough compared with 10pg samples?

Reads information

Input ng # N2.id01_avg N2.id02_avg N2.id03_avg N2.id04_avg
0 2 0.18% 0.16% 0.81% 0.54%
0.1 12 2.58% 2.63% 3.60% 3.48%
1 2 34.36% 34.07% 27.62% 28.60%
total Reads 3,146,372 2,366,588 4,194,796 3,431,696
Input ng # N2.id05_avg N2.id06_avg N2.id07_avg N2.id08_avg
0 2 0.70% 0.84% 3.47% 3.53%
0.01 12 2.76% 2.81% 5.26% 5.24%
0.1 2 32.73% 32.32% 14.95% 15.03%
total reads 3,483,059 1,668,975 5,572,495 1,774,114