Rui:RNAseq analysis on 121226 HL140: Difference between revisions
Jump to navigation
Jump to search
>RuiLiu (Created page with "==RNAseq on 121226_HL140== * Continue with Kun's note [http://genome-tech.ucsd.edu/LabNotes/index.php/Kun:LabNotes/SingleCellExpr/2013-1-1] * A number of questions that I hope...") |
>RuiLiu |
||
Line 24: | Line 24: | ||
|- | |- | ||
| total Reads||||3,146,372||2,366,588||4,194,796||3,431,696 | | total Reads||||3,146,372||2,366,588||4,194,796||3,431,696 | ||
| | |} | ||
| | |||
| | {| {{table}} border=1 | ||
| Input ng||#||N2.id05_avg||N2.id06_avg||N2.id07_avg||N2.id08_avg | | align="center" style="background:#f0f0f0;"|'''Input ng''' | ||
| align="center" style="background:#f0f0f0;"|'''#''' | |||
| align="center" style="background:#f0f0f0;"|'''N2.id05_avg''' | |||
| align="center" style="background:#f0f0f0;"|'''N2.id06_avg''' | |||
| align="center" style="background:#f0f0f0;"|'''N2.id07_avg''' | |||
| align="center" style="background:#f0f0f0;"|'''N2.id08_avg''' | |||
|- | |- | ||
| 0||2||0.70%||0.84%||3.47%||3.53% | | 0||2||0.70%||0.84%||3.47%||3.53% |
Revision as of 00:02, 3 January 2013
RNAseq on 121226_HL140
- Continue with Kun's note [1]
- A number of questions that I hope to answer with this data set include:
- Is there any indication that we can obtain reasonable data from 10pg of input total RNA?
- Is there a difference in efficiency between TSOs with and without the 8bp barcodes?
- Is there a difference in efficiency between T20V and T20VN primer?
- Is there a difference in efficiency between 72C and 10C annealing?
- Is NTC clean enough compared with 10pg samples?
Reads information
Input ng | # | N2.id01_avg | N2.id02_avg | N2.id03_avg | N2.id04_avg |
0 | 2 | 0.18% | 0.16% | 0.81% | 0.54% |
0.1 | 12 | 2.58% | 2.63% | 3.60% | 3.48% |
1 | 2 | 34.36% | 34.07% | 27.62% | 28.60% |
total Reads | 3,146,372 | 2,366,588 | 4,194,796 | 3,431,696 |
Input ng | # | N2.id05_avg | N2.id06_avg | N2.id07_avg | N2.id08_avg |
0 | 2 | 0.70% | 0.84% | 3.47% | 3.53% |
0.01 | 12 | 2.76% | 2.81% | 5.26% | 5.24% |
0.1 | 2 | 32.73% | 32.32% | 14.95% | 15.03% |
total reads | 3,483,059 | 1,668,975 | 5,572,495 | 1,774,114 |