Ho Suk Lee: Difference between revisions

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==Fluorescent in situ RNA sequencing(FISSEQ)==
==Fluorescent in situ RNA sequencing(FISSEQ)==
*Project goal: Development of a highly multiplexed (hundreds of transcripts to full transcriptome) in situ RNA sequencing method for the determination of transcriptional profiles of single cells in structured tissues (primary neurons and cortex sections).
*[[Hosuk:Background|Background information]]
*[[Hosuk:Background|Background information]]
*[[Hosuk:Lab Notes|Lab Notes]]
*[[Hosuk:Lab Notes|Lab Notes]]
*[[Hosuk:FISSEQ Primer information|FISSEQ Primer, Probe information]]
*[[Hosuk:Instruments information|Instruments information]]
*[[Hosuk:Instruments information|Instruments information]]
==MDA Microfluidic device==
*Chromosome separation, amplification and tagmentation
*[[Hosuk:Lab Notes MDA|Lab Notes]]
*[[Media:Singel Cell Genome MDA Tagmentation_DeviceHistory_v1.2.pdf|Device History (v1.2)]]




==Protocols==
==Protocols==
*[[Hosuk:Protocols|Protocols]]
*FISSEQ
** [[Media:DARTFISH Protocol v1.0.pdf|DARTFISH]]
** [[Hosuk:cDNA Rolony construction|cDNA rolony]]
** [[Hosuk:Cell fixation in culture dish|Cell fixation in culture dish]]
 
 
*MDA Microfluidic device
** [[Hosuk:MDA Microfluidic device fabrication|PDMS device fabrication]]
** [[Hosuk:MDA Master mix|MDA Master mix]]
 


*Tagmentation (from Jeff)
** [[Hosuk:Tagmentation Protocol|Tagmentation (edited at 2013-07-23)]]


==Labmeeting Presentation==
==Labmeeting Presentation==
*[[Media:Lab meeting PT - 080812_HoSuk.pptx|2012-08-12]]
*[[Hosuk:Labmeeting2015|2015]]
*[[Media:Lab meeting PT - 102912_HoSuk.pptx|2012-10-29]]
*[[Hosuk:Labmeeting2014|2014]]
*[[Media:Lab meeting PT - 011513_HoSuk.pptx|2013-01-15]]
*[[Hosuk:Labmeeting2013|2013]]
*[[Hosuk:Labmeeting2012|2012]]

Latest revision as of 18:26, 18 June 2015