Leica SP8 Confocal Microscope: Difference between revisions

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===Leica support contact===
===Leica support contact===
*Technical support: (Toll Free) 866-830-0735
#Technical support: (Toll Free) 866-830-0735
 
#Clark Thom
#*West Coast Team Leader Field Service
#*619-278-8324
#*clark.thom@leica-microsystems.com


*Clark Thom
#Carlos Alonso
West Coast Team Leader Field Service
#*Confocal Applications Specialist
619-278-8324
#*858-353-8062
clark.thom@leica-microsystems.com
#*carlos.alonso@leica-microsystems.com


*Carlos Alonso
*(Call technical support first before trying to contact Clark)
Confocal Applications Specialist
858-353-8062
carlos.alonso@leica-microsystems.com





Revision as of 22:38, 16 January 2013

Location

PFBH 036

File:Confocal01.jpg File:Confocal02.jpg


Leica support contact

  1. Technical support: (Toll Free) 866-830-0735
  1. Clark Thom
    • West Coast Team Leader Field Service
    • 619-278-8324
    • clark.thom@leica-microsystems.com
  1. Carlos Alonso
    • Confocal Applications Specialist
    • 858-353-8062
    • carlos.alonso@leica-microsystems.com
  • (Call technical support first before trying to contact Clark)


General specification

  1. 3 Laser source – 488, 552, 638nm, Solid State, up to 20mW
  2. 2 PMT (Photo Multiplier Tube)
  3. 3 Objective Lens
    • 10x NA = 0.3
    • 20x NA = 0.75(Glycerine or Oil immersion)
    • 63x NA=1.4(Oil immersion)
  4. Z-axis step size – 50nm
  5. Scan area - 1.16 mm x 1.16mm (@10x obj., zoom = 1x) ~ 24.22 um x 24.22um (@10x obj., zoom = 48x)
  6. Image size - up to 8192 x 8192 pixel
    • 1 pixel = 141.92nm @10x obj., 22.53nm @ 63x obj.
    • Max. Scan field : 21.2mm (Tile scanning)


Sample preparation

  • Only one important consideration is that substrate must be #1.5 glass slide!

Starting and Finishing sequence