Leica SP8 Confocal Microscope: Difference between revisions
Jump to navigation
Jump to search
>Hosuki78 No edit summary |
>Hosuki78 No edit summary |
||
Line 7: | Line 7: | ||
| [[File:Confocal02.jpg|400px]] | | [[File:Confocal02.jpg|400px]] | ||
|} | |} | ||
===Leica support contact=== | ===Leica support contact=== | ||
#Technical support: (Toll Free) 866-830-0735 | |||
#Clark Thom | |||
#*West Coast Team Leader Field Service | |||
#*619-278-8324 | |||
#*clark.thom@leica-microsystems.com | |||
* | #Carlos Alonso | ||
#*Confocal Applications Specialist | |||
#*858-353-8062 | |||
#*carlos.alonso@leica-microsystems.com | |||
* | *(Call technical support first before trying to contact Clark) | ||
Revision as of 22:38, 16 January 2013
Location
PFBH 036
File:Confocal01.jpg | File:Confocal02.jpg |
Leica support contact
- Technical support: (Toll Free) 866-830-0735
- Clark Thom
- West Coast Team Leader Field Service
- 619-278-8324
- clark.thom@leica-microsystems.com
- Carlos Alonso
- Confocal Applications Specialist
- 858-353-8062
- carlos.alonso@leica-microsystems.com
- (Call technical support first before trying to contact Clark)
General specification
- 3 Laser source – 488, 552, 638nm, Solid State, up to 20mW
- 2 PMT (Photo Multiplier Tube)
- 3 Objective Lens
- 10x NA = 0.3
- 20x NA = 0.75(Glycerine or Oil immersion)
- 63x NA=1.4(Oil immersion)
- Z-axis step size – 50nm
- Scan area - 1.16 mm x 1.16mm (@10x obj., zoom = 1x) ~ 24.22 um x 24.22um (@10x obj., zoom = 48x)
- Image size - up to 8192 x 8192 pixel
- 1 pixel = 141.92nm @10x obj., 22.53nm @ 63x obj.
- Max. Scan field : 21.2mm (Tile scanning)
Sample preparation
- Only one important consideration is that substrate must be #1.5 glass slide!