Leica SP8 Confocal Microscope: Difference between revisions
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===Location=== | ===Location=== | ||
PFBH 036 | PFBH 036 | ||
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*Only one important consideration is that substrate must be '''#1.5 glass slide'''! | *Only one important consideration is that substrate must be '''#1.5 glass slide'''! | ||
===Workstation=== | |||
*(This workstation has dynamic IP address, so the IP information below can be changed) | |||
*IP address : 132.239.236.163 | |||
*Subnet Mask : 255.255.255.0 | |||
*Gateway : 132.239.236.1 | |||
*DNS : 132.239.1.19 | |||
*MAC : B4:B5:2F:CC:91:7F | |||
*Network Port: B.1.32 (Room #038) | |||
*Login: | |||
**admin: !Admin12345 | |||
**ConfocalUser: True!Confocal55 | |||
===Turning on/off sequence=== | ===Turning on/off sequence=== | ||
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| [[File:ConfocalPower_TurnOff.jpg|250px]] | | [[File:ConfocalPower_TurnOff.jpg|250px]] | ||
|} | |} | ||
Latest revision as of 20:49, 18 November 2016
Location[edit]
PFBH 036
Leica support contact[edit]
- Technical support: (Toll Free) 866-830-0735
- Clark Thom
- West Coast Team Leader Field Service
- 619-278-8324
- clark.thom@leica-microsystems.com
- Carlos Alonso
- Confocal Applications Specialist
- 858-353-8062
- carlos.alonso@leica-microsystems.com
- (Call technical support first before trying to contact Clark)
General specification[edit]
- 3 Laser source – 488, 552, 638nm, Solid State, up to 20mW
- 2 PMT (Photo Multiplier Tube)
- 3 Objective Lens
- 10x NA = 0.3
- 20x NA = 0.75(Glycerine or Oil immersion)
- 63x NA=1.4(Oil immersion)
- Z-axis step size – 50nm
- Scan area - 1.16 mm x 1.16mm (@10x obj., zoom = 1x) ~ 24.22 um x 24.22um (@10x obj., zoom = 48x)
- Image size - up to 8192 x 8192 pixel
- 1 pixel = 141.92nm @10x obj., 22.53nm @ 63x obj.
- Max. Scan field : 21.2mm (Tile scanning)
Sample preparation[edit]
- Only one important consideration is that substrate must be #1.5 glass slide!
Workstation[edit]
- (This workstation has dynamic IP address, so the IP information below can be changed)
- IP address : 132.239.236.163
- Subnet Mask : 255.255.255.0
- Gateway : 132.239.236.1
- DNS : 132.239.1.19
- MAC : B4:B5:2F:CC:91:7F
- Network Port: B.1.32 (Room #038)
- Login:
- admin: !Admin12345
- ConfocalUser: True!Confocal55
Turning on/off sequence[edit]
- Turn on sequence
- Confocal Power supply : Turn on from left to right Scan power --> Laser power --> Key (turn clockwise)
- PC power strip : Turn on the switch on the strip
- Turn off sequence --> Reverse way of turn on sequence
- Shut down PC
- Turn off PC power strip
- Turn off confocal power supply : Turn off from right to left : Key --> Laser power --> Scan power
File:ConfocalPower TurnOn.jpg | File:ConfocalPower TurnOff.jpg |