Jie:LabNotes/sample record/sample received: Difference between revisions
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>Jie deng (New page: 2008-07-24: From James Thomson, Biotechnology Center, Madison, WI. Two tubes tube1:IMR90 tube2:IMR90-04 2008-08-01: From James Thomson,Biotechnology Center, Madison, WI. Two tubes. T...) |
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2008-07-24: From James Thomson, Biotechnology Center, Madison, WI. Two tubes | 2008-07-24: From James Thomson, Biotechnology Center, Madison, WI. Two tubes | ||
tube1:IMR90 | tube1:IMR90 | ||
tube2:IMR90-04 | tube2:IMR90-04 | ||
2008-08-01: From James Thomson,Biotechnology Center, Madison, WI. Two tubes. | |||
2008-08-01: From James Thomson,Biotechnology Center, Madison, WI. Two tubes. | |||
Tube 1: IMR90P24, 7/29/08 | Tube 1: IMR90P24, 7/29/08 | ||
tube 2: Qwell of le well plate, ipsIMR90CH, P28,18,4, 7/22/08 | tube 2: Qwell of le well plate, ipsIMR90CH, P28,18,4, 7/22/08 | ||
20080826: From Paul Ventura, Covaris, Inc. refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2008-8-12] | |||
IMB90_iPS_sheared sequencing DNA. Nanodrop:dsDNA 19.8ng/ul; 260/280 1.07; 260/230 0.51 | |||
Hybrid_1_sheared sequencing DNA. Nanodrop:dsDNA 21.6ng/ul; 260/280 1.13; 260/230 0.56 | |||
20090515:From Yuan Gao | |||
E0H 45ng/ul 50ul; | |||
E4H 45ng/ul 50ul; | |||
E24H 50ng/ul 42ul; | |||
20090518 from Kun | |||
DF iPS from Thomson lab refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2009-5-29] | |||
foreskin 1.0 583ng/ul 1.93 1.16 | |||
DF6-9-9 P16(4) 0.3 504.8ng/ul 1.88 2.04 | |||
DF4-5-7 P16(4) 0.4 541ng/ul 1.86 1.99 | |||
DF6-9-12 p16(4) 0.4 425.3ng/ul 1.88 2.09 | |||
DF19-9-11 p17(4) 0.4 484.7ng/ul 1.89 2.04 | |||
DF19-9-7 p17(4) 0.3 460.3ng/ul 1.89 2.04 | |||
BJ 60ng/ul x 200ul | |||
BJ-iPS11 140ng/ul x 30ul | |||
BJ-iPS12 40ng/ul x 80ul | |||
HUES6 250ng/ul x 200ul | |||
NA21687*1 062507 241.1ng/ul 1.89 1.51 refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2009-6-19] | |||
NA21781*1 102307 240ng/ul 1.89 1.26 | |||
NA21833*1 101807 253.9ng/ul 1.9 1.49 | |||
NA21660*1 053007 243.2ng/ul 1.92 1.66 | |||
NA21731*1 101807 217.4ng/ul 1.88 1.49 | |||
NA21846*1 100407 186.5ng/ul 1.89 1.47 | |||
NA21070*1 100406 329.7ng/ul 1.99 1.77 | |||
cell: | |||
hiPS 11 P17 | |||
hiPs 12 P20 | |||
2009-06-17: From Zhuohua Zhang. refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2009-7-1] | |||
No 5028-1818 are unaffacted control tissues; | |||
No 1947-4526 are Parkinson's disease and Parkinson's disease/Lewy body disease. | |||
5028: 320ng/ul; | |||
4735: 260ng/ul; | |||
4789: 280ng/ul; | |||
5171: 420ng/ul; | |||
1818: 480ng/ul; | |||
1947: 360ng/ul; | |||
1741: 560ng/ul; | |||
4977: 260ng/ul; | |||
4879: 340ng/ul; | |||
4526: 380ng/ul. | |||
[[Image:20090617_gDNA from Zhuohua Zhang.jpg|200px]]20090617_gDNA from Zhuohua Zhang | |||
2009-07-28: From kim Smoga-Otto of Thomson lab.refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2009-8-10] | |||
two tubes of iPS-DF6.9h, passage 28. (17 Jul 09). 5 million cell/tube. | |||
2009-07-28: From Jehyuk Lee from Harvard Medical school. | |||
PGP1_iPS1 gDNA: 47ng/ul x 50ul; | |||
PGP1_iPS1 mRNA: 476ng/ul x 20ul; | |||
PGP1_iPS1 ssDNA from mRNA: 146ng/ul x 10ul. | |||
2009-08-04: From Kun (breast cancer cells from patient) refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2009-8-7] | |||
05192A18 | |||
05192B09 | |||
2009-09-04: From | |||
LD 353-03 53.7ng/ul | |||
LD351-01 73.6ng/ul | |||
MEC001 47.8ng/ul | |||
KaG001 31.9ng/ul | |||
2009-10: from Harvard | |||
{| border="1" cellpadding="5" cellspacing="0" align="center" | |||
|- | |||
| align="center" style="background:#f0f0f0;"|''' ''' | |||
| align="center" style="background:#f0f0f0;"|'''sample ''' | |||
|align="center" style="background:#f0f0f0;"|'''sample concentration''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280, 260/230''' | |||
| align="center" style="background:#f0f0f0;"|'''volume ''' | |||
|- | |||
|Harvard_1||B0047.1 ||927.1ng/ul||1.8/2.25||20ul | |||
|- | |||
|Harvard_2||B0060.1||206ng/ul ||1.92/1.56||20ul | |||
|- | |||
|Harvard_3||B0070.1||760ng/ul||1.9/2.1||20ul | |||
|- | |||
|Harvard_4||B0107.1||360ng/ul ||1.9/1.9||5ul | |||
|- | |||
|Harvard_5||B0108.1 ||266ng/ul ||1.9/1.5||15ul | |||
|- | |||
|Harvard_6||B0134.1||54.2ng/ul||1.9/1.2||40ul | |||
|- | |||
|Harvard_7||M0013.1 ||380ng/ul ||1.9/2.0||20ul | |||
|- | |||
|Harvard_8||M0077.3||47ng/ul ||1.9/1.2||40ul | |||
|- | |||
|Harvard_9||M0124.3 ||46ng/ul||1.9/1.1||40ul | |||
|- | |||
|Harvard_10||M0188.3||82ng/ul||1.9/1.6||30ul | |||
|- | |||
|} | |||
seq lib construction refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2010-1-21] and [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2010-1-28] | |||
the sample sequencing tracking can be seen [[Media:20100210_harvard_sample_sequencing_tracking.xls]] | |||
20090918 from cancer center | |||
The sample tracking can be seen [[Media:20090918_breast_cancer_samples.xls]] | |||
20100121 from Y. Geno Shi in Harvard. refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/CpgSeq/2010-1-22] | |||
ct1 mES gDNA 11.20.09: 120ng/ul | |||
Tet1-KD mES gDNA 11.20.09: 120ng/ul |
Latest revision as of 00:17, 19 February 2010
2008-07-24: From James Thomson, Biotechnology Center, Madison, WI. Two tubes tube1:IMR90 tube2:IMR90-04
2008-08-01: From James Thomson,Biotechnology Center, Madison, WI. Two tubes. Tube 1: IMR90P24, 7/29/08 tube 2: Qwell of le well plate, ipsIMR90CH, P28,18,4, 7/22/08
20080826: From Paul Ventura, Covaris, Inc. refer to LabNotes on [1] IMB90_iPS_sheared sequencing DNA. Nanodrop:dsDNA 19.8ng/ul; 260/280 1.07; 260/230 0.51 Hybrid_1_sheared sequencing DNA. Nanodrop:dsDNA 21.6ng/ul; 260/280 1.13; 260/230 0.56
20090515:From Yuan Gao E0H 45ng/ul 50ul; E4H 45ng/ul 50ul; E24H 50ng/ul 42ul;
20090518 from Kun DF iPS from Thomson lab refer to LabNotes on [2]
foreskin 1.0 583ng/ul 1.93 1.16 DF6-9-9 P16(4) 0.3 504.8ng/ul 1.88 2.04 DF4-5-7 P16(4) 0.4 541ng/ul 1.86 1.99 DF6-9-12 p16(4) 0.4 425.3ng/ul 1.88 2.09 DF19-9-11 p17(4) 0.4 484.7ng/ul 1.89 2.04 DF19-9-7 p17(4) 0.3 460.3ng/ul 1.89 2.04
BJ 60ng/ul x 200ul BJ-iPS11 140ng/ul x 30ul BJ-iPS12 40ng/ul x 80ul
HUES6 250ng/ul x 200ul
NA21687*1 062507 241.1ng/ul 1.89 1.51 refer to LabNotes on [3] NA21781*1 102307 240ng/ul 1.89 1.26 NA21833*1 101807 253.9ng/ul 1.9 1.49 NA21660*1 053007 243.2ng/ul 1.92 1.66 NA21731*1 101807 217.4ng/ul 1.88 1.49 NA21846*1 100407 186.5ng/ul 1.89 1.47 NA21070*1 100406 329.7ng/ul 1.99 1.77
cell: hiPS 11 P17 hiPs 12 P20
2009-06-17: From Zhuohua Zhang. refer to LabNotes on [4] No 5028-1818 are unaffacted control tissues; No 1947-4526 are Parkinson's disease and Parkinson's disease/Lewy body disease. 5028: 320ng/ul; 4735: 260ng/ul; 4789: 280ng/ul; 5171: 420ng/ul; 1818: 480ng/ul; 1947: 360ng/ul; 1741: 560ng/ul; 4977: 260ng/ul; 4879: 340ng/ul; 4526: 380ng/ul. File:20090617 gDNA from Zhuohua Zhang.jpg20090617_gDNA from Zhuohua Zhang
2009-07-28: From kim Smoga-Otto of Thomson lab.refer to LabNotes on [5] two tubes of iPS-DF6.9h, passage 28. (17 Jul 09). 5 million cell/tube.
2009-07-28: From Jehyuk Lee from Harvard Medical school. PGP1_iPS1 gDNA: 47ng/ul x 50ul; PGP1_iPS1 mRNA: 476ng/ul x 20ul; PGP1_iPS1 ssDNA from mRNA: 146ng/ul x 10ul.
2009-08-04: From Kun (breast cancer cells from patient) refer to LabNotes on [6] 05192A18 05192B09
2009-09-04: From LD 353-03 53.7ng/ul LD351-01 73.6ng/ul MEC001 47.8ng/ul KaG001 31.9ng/ul
2009-10: from Harvard
sample | sample concentration | 260/280, 260/230 | volume | |
Harvard_1 | B0047.1 | 927.1ng/ul | 1.8/2.25 | 20ul |
Harvard_2 | B0060.1 | 206ng/ul | 1.92/1.56 | 20ul |
Harvard_3 | B0070.1 | 760ng/ul | 1.9/2.1 | 20ul |
Harvard_4 | B0107.1 | 360ng/ul | 1.9/1.9 | 5ul |
Harvard_5 | B0108.1 | 266ng/ul | 1.9/1.5 | 15ul |
Harvard_6 | B0134.1 | 54.2ng/ul | 1.9/1.2 | 40ul |
Harvard_7 | M0013.1 | 380ng/ul | 1.9/2.0 | 20ul |
Harvard_8 | M0077.3 | 47ng/ul | 1.9/1.2 | 40ul |
Harvard_9 | M0124.3 | 46ng/ul | 1.9/1.1 | 40ul |
Harvard_10 | M0188.3 | 82ng/ul | 1.9/1.6 | 30ul |
seq lib construction refer to LabNotes on [7] and [8] the sample sequencing tracking can be seen Media:20100210_harvard_sample_sequencing_tracking.xls
20090918 from cancer center The sample tracking can be seen Media:20090918_breast_cancer_samples.xls
20100121 from Y. Geno Shi in Harvard. refer to LabNotes on [9] ct1 mES gDNA 11.20.09: 120ng/ul Tet1-KD mES gDNA 11.20.09: 120ng/ul