Hosuk:Cell fixation in culture dish: Difference between revisions

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>Hosuki78
(Created page with "#200uL of formalin (~39% formaldehyde)directly to 2 ml of culture dish, incubate for 15min at 37C #Change media of fresh formalin+PBS, incubate for 15min at 37C, #Wash with PB...")
 
>Hosuki78
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#200uL of formalin (~39% formaldehyde)directly to 2 ml of culture dish, incubate for 15min at 37C
#For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
#Change media of fresh formalin+PBS, incubate for 15min at 37C,
#1mL of formalin (~39% formaldehyde)directly to 2 ml of culture dish, incubate for 15min at 37C
#Wash with PBS, and add 70% Ethanol  
#Change media of fresh formalin+PBS (1mL), incubate for 15min at 37C,
#Wash with PBS, and add 70% Ethanol (2mL)
#Store at 4C
#Store at 4C

Revision as of 19:06, 30 January 2013

  1. For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
  2. 1mL of formalin (~39% formaldehyde)directly to 2 ml of culture dish, incubate for 15min at 37C
  3. Change media of fresh formalin+PBS (1mL), incubate for 15min at 37C,
  4. Wash with PBS, and add 70% Ethanol (2mL)
  5. Store at 4C