Hosuk:Cell fixation in culture dish: Difference between revisions

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#For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
*For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
#1mL of formalin (~39% formaldehyde)directly to 2 ml of culture dish, incubate for 15min at 37C
#1mL of formalin (~39% formaldehyde)directly to 2 ml of culture dish, incubate for 15min at 37C
#Change media of fresh formalin+PBS (1mL), incubate for 15min at 37C,
#Change media of fresh formalin+PBS (1mL), incubate for 15min at 37C,
#Wash with PBS, and add 70% Ethanol (2mL)  
#Wash with PBS, and add 70% Ethanol (2mL)  
#Store at 4C
#Store at 4C

Revision as of 19:07, 30 January 2013

  • For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
  1. 1mL of formalin (~39% formaldehyde)directly to 2 ml of culture dish, incubate for 15min at 37C
  2. Change media of fresh formalin+PBS (1mL), incubate for 15min at 37C,
  3. Wash with PBS, and add 70% Ethanol (2mL)
  4. Store at 4C