Hosuk:Cell fixation in culture dish: Difference between revisions
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*For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C) | *For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C) | ||
# | #NO aspirate media. add 200 ul formalin(~39% formaldehyde) to 2 ml media directly, | ||
# | #incubate for 15 min at 37C | ||
# | #aspirate | ||
#quickly add pre warmed 10% formalin in PBS | |||
#incubate at 37C for 15 min | |||
#aspirate | |||
#wash with PBS 3 times at RT | |||
#incubate in 70% etoh for 1 min or store in 70% etoh up to a week at 4C | |||
#Store at 4C | #Store at 4C |
Revision as of 17:57, 11 February 2013
- For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
- NO aspirate media. add 200 ul formalin(~39% formaldehyde) to 2 ml media directly,
- incubate for 15 min at 37C
- aspirate
- quickly add pre warmed 10% formalin in PBS
- incubate at 37C for 15 min
- aspirate
- wash with PBS 3 times at RT
- incubate in 70% etoh for 1 min or store in 70% etoh up to a week at 4C
- Store at 4C