Hosuk:Cell fixation in culture dish: Difference between revisions

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*For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
*For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
#200uL of formalin (~39% formaldehyde)directly, NO aspirate media, incubate for 15min at 37C
#NO aspirate media. add 200 ul formalin(~39% formaldehyde) to 2 ml media directly,  
#Change media of fresh formalin+PBS (No wash!), incubate for 15min at 37C,
#incubate for 15 min at 37C
#Wash with PBS three times, and add 70% Ethanol (2mL)
#aspirate
#quickly add pre warmed 10% formalin in PBS
#incubate at 37C for 15 min
#aspirate
#wash with PBS 3 times at RT
#incubate in 70% etoh for 1 min or store in 70% etoh up to a week at 4C
#Store at 4C
#Store at 4C

Revision as of 17:57, 11 February 2013

  • For cells in MatTek glass bottom dish(P/N : P35G-1.5-14-C)
  1. NO aspirate media. add 200 ul formalin(~39% formaldehyde) to 2 ml media directly,
  2. incubate for 15 min at 37C
  3. aspirate
  4. quickly add pre warmed 10% formalin in PBS
  5. incubate at 37C for 15 min
  6. aspirate
  7. wash with PBS 3 times at RT
  8. incubate in 70% etoh for 1 min or store in 70% etoh up to a week at 4C
  9. Store at 4C