EricChu:Protocols: Difference between revisions

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>Ericchu
(Created page with "==RephiPHI MDA Protocol (20ul reaction)== #Start with 1.0ul λ DNA (2.5ng/ul) #Add 1.5ul ALS, incubate at room temp 3mins #Add 1.5ul NS, put on ice immediately #Create master...")
 
>Ericchu
(Blanked the page)
 
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==RephiPHI MDA Protocol (20ul reaction)==


#Start with 1.0ul λ DNA (2.5ng/ul)
#Add 1.5ul ALS, incubate at room temp 3mins
#Add 1.5ul NS, put on ice immediately
#Create master mix (w/ 1% Tween20)
##2ul 10x buffer
##5ul 200uM N6 primers
##0.8ul 25mM dNTPs
##2ul Phi29 polymerase
##2ul 8% Tween20
##4.2ul H20
ALS: 69.5ul H20 + 10ul DTT(1M) + 8ul KOH (5M) + 12.5ul 8% Tween20
NS: 87.5ul NS stock + 12.5ul 8% Tween20
where NS stock: 4ml 1MHCl and 6ml 1MTrisHCl

Latest revision as of 23:28, 4 February 2013