Jie:LabNotes/oligo sequences: Difference between revisions

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   confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K.
   confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K.
   [[Media: 20080918_conirmed primers for cpg30k.xls]]
   [[Media: 20080918_conirmed primers for cpg30k.xls]]
20080929
  sapcer with 60mer that can be ligated without extension.
  Spacer_60mer_reverse: gacctcgataacatctgcagTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGCTGAATAGGAACGATGAGCC
  Spacer_60mer: 5’-Phos/ctggaaggattctcaccaaacgcaacggtgattgtactggttctggtgttctactttgca

Revision as of 19:11, 29 September 2008

20080716

 primer to produce spacer:refer to LabNotes on [1]

CirclehelperV4_short(20080825):

 AACAGTGCTCTTCCAGTCTACTAGCCTCATGCGTATCCGATC

20080904

 Prmer used to amplify the cpg30k and cosmic3760. The amplicon will have 5-terminal phosphate, thus can be ligated after circlehelper insertion.
 AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC
 AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC

20080918

 confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K.
 Media: 20080918_conirmed primers for cpg30k.xls

20080929

 sapcer with 60mer that can be ligated without extension.
 Spacer_60mer_reverse: gacctcgataacatctgcagTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGCTGAATAGGAACGATGAGCC
 Spacer_60mer: 5’-Phos/ctggaaggattctcaccaaacgcaacggtgattgtactggttctggtgttctactttgca