Jie:LabNotes/oligo sequences: Difference between revisions
Jump to navigation
Jump to search
>Jie deng No edit summary |
>Jie deng No edit summary |
||
Line 15: | Line 15: | ||
20080929 | 20080929 | ||
sapcer with 60mer that can be ligated without extension. | sapcer with length of 60mer that can be ligated without extension. | ||
Spacer_60mer_reverse: gacctcgataacatctgcagTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGCTGAATAGGAACGATGAGCC | Spacer_60mer_reverse: gacctcgataacatctgcagTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGCTGAATAGGAACGATGAGCC | ||
Spacer_60mer: 5’-Phos/ctggaaggattctcaccaaacgcaacggtgattgtactggttctggtgttctactttgca | Spacer_60mer: 5’-Phos/ctggaaggattctcaccaaacgcaacggtgattgtactggttctggtgttctactttgca | ||
[[Media:20080929_spacer of 60mer. | [[Media:20080929_spacer of 60mer.doc]] |
Revision as of 16:44, 19 October 2008
20080716
primer to produce spacer:refer to LabNotes on [1]
CirclehelperV4_short(20080825):
AACAGTGCTCTTCCAGTCTACTAGCCTCATGCGTATCCGATC
20080904
Prmer used to amplify the cpg30k and cosmic3760. The amplicon will have 5-terminal phosphate, thus can be ligated after circlehelper insertion. AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC
20080918
confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K. Media: 20080918_conirmed primers for cpg30k.xls
20080929
sapcer with length of 60mer that can be ligated without extension. Spacer_60mer_reverse: gacctcgataacatctgcagTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGCTGAATAGGAACGATGAGCC Spacer_60mer: 5’-Phos/ctggaaggattctcaccaaacgcaacggtgattgtactggttctggtgttctactttgca Media:20080929_spacer of 60mer.doc