Jie:LabNotes/oligo sequences: Difference between revisions

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   confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K.
   confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K.
   [[Media: 20080918_conirmed primers for cpg30k.xls]]
   [[Media: 20080918_conirmed primers for cpg30k.xls]]
20080929
  sapcer with length of 60mer that can be ligated without extension.
  Spacer_60mer_reverse: gacctcgataacatctgcagTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGCTGAATAGGAACGATGAGCC
  Spacer_60mer: 5’-Phos/ctggaaggattctcaccaaacgcaacggtgattgtactggttctggtgttctactttgca
  [[Media:20080929_spacer of 60mer.doc]]
20081019
  confirmation primers for cpg30k from cpg28k library.
  [[Media: 20081019_conirmed primers for cpg30k from cpg28k.xls]]
20081019
  positive control used for cosmic probe circlehelper insertion.
  cosmic_probe_control: GTAGACTGGAAGAGCACTGTTGTTGGAGGCTCATCGTTCCTATTCAGCTGCAGATGTTATCGAGGTCCGACGATCGGATACGCATGAGGCTA
20081024
  more confirmation primers for cpg30k from cpg28k library.
  [[Media: 20081024_confirmed primers plate_2 for cpg30k from cpg28k.xls]]
  [[Media: 20081024_conirmed primers plate_3 for cpg30k from cpg28k.xls]]

Latest revision as of 22:29, 25 November 2008

20080716

 primer to produce spacer:refer to LabNotes on [1]

CirclehelperV4_short(20080825):

 AACAGTGCTCTTCCAGTCTACTAGCCTCATGCGTATCCGATC

20080904

 Prmer used to amplify the cpg30k and cosmic3760. The amplicon will have 5-terminal phosphate, thus can be ligated after circlehelper insertion.
 AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC
 AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC

20080918

 confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K.
 Media: 20080918_conirmed primers for cpg30k.xls

20080929

 sapcer with length of 60mer that can be ligated without extension.
 Spacer_60mer_reverse: gacctcgataacatctgcagTGCAAAGTAGAACACCAGAACCAGTACAATCACCGTTGCGTTTGGTGAGAATCCTTCCAGCTGAATAGGAACGATGAGCC
 Spacer_60mer: 5’-Phos/ctggaaggattctcaccaaacgcaacggtgattgtactggttctggtgttctactttgca
 Media:20080929_spacer of 60mer.doc

20081019

 confirmation primers for cpg30k from cpg28k library. 
 Media: 20081019_conirmed primers for cpg30k from cpg28k.xls

20081019

 positive control used for cosmic probe circlehelper insertion.
 cosmic_probe_control: GTAGACTGGAAGAGCACTGTTGTTGGAGGCTCATCGTTCCTATTCAGCTGCAGATGTTATCGAGGTCCGACGATCGGATACGCATGAGGCTA

20081024

 more confirmation primers for cpg30k from cpg28k library. 
 Media: 20081024_confirmed primers plate_2 for cpg30k from cpg28k.xls
 Media: 20081024_conirmed primers plate_3 for cpg30k from cpg28k.xls