Noi/NOTES/2013-2-27: Difference between revisions

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* [[http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes '''Link to calendar''']]
* [[http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes '''Link to calendar''']]
== Plan for probe preparation for Matt's project ==
== Plan for probe preparation for Matt's project ==
* I will be preparing probes using the same method as I did for LC Sciences oligoes, [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2011-10-6| LC_Sciences probe prep]]
* I will be preparing probes using the same method as I did for LC Sciences oligoes, [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2011-10-6]]
* Check reagents to make sure I have all reagents ready for probe prep and padlock capture
* Check reagents to make sure I have all reagents ready for probe prep and padlock capture
  '''Probe prep'''
  '''Probe prep'''

Revision as of 00:13, 1 March 2013

Plan for probe preparation for Matt's project

  • I will be preparing probes using the same method as I did for LC Sciences oligoes, [[1]]
  • Check reagents to make sure I have all reagents ready for probe prep and padlock capture
Probe prep
- Waiting for seed oligo: expected to come next Wednesday (March6, 2013)
- eMIP_CA1_F and eMIP_CA1_R primer for expansion PCR and production PCR (very little left, ordered 2/28/2013)
- 2x KAPA SYBG FAST qPCR MM
- Nt.AlwI (NEB) and Nb.BsrDI (NEB) -> ordered 2/26/2013
- Qiaquick colums, TBU gel, Nanosep column
Padlock capture
Note: There is no gap for this capture, so only need Ampligase
- Ampligase and buffer
- ExoI/ExoIII
- Amplification primers (Matt)