Noi/NOTES/2013-2-27: Difference between revisions
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* [[http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes '''Link to calendar''']] | * [[http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes '''Link to calendar''']] | ||
== Plan for probe preparation for Matt's project == | == Plan for probe preparation for Matt's project == | ||
* I will be preparing probes using the same method as I did for LC Sciences oligoes, [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2011-10-6 | * I will be preparing probes using the same method as I did for LC Sciences oligoes, [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2011-10-6]] | ||
* Check reagents to make sure I have all reagents ready for probe prep and padlock capture | * Check reagents to make sure I have all reagents ready for probe prep and padlock capture | ||
'''Probe prep''' | '''Probe prep''' |
Revision as of 00:13, 1 March 2013
Plan for probe preparation for Matt's project
- I will be preparing probes using the same method as I did for LC Sciences oligoes, [[1]]
- Check reagents to make sure I have all reagents ready for probe prep and padlock capture
Probe prep - Waiting for seed oligo: expected to come next Wednesday (March6, 2013) - eMIP_CA1_F and eMIP_CA1_R primer for expansion PCR and production PCR (very little left, ordered 2/28/2013) - 2x KAPA SYBG FAST qPCR MM - Nt.AlwI (NEB) and Nb.BsrDI (NEB) -> ordered 2/26/2013 - Qiaquick colums, TBU gel, Nanosep column Padlock capture Note: There is no gap for this capture, so only need Ampligase - Ampligase and buffer - ExoI/ExoIII - Amplification primers (Matt)