03112013: Difference between revisions
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Purpose: Increase fragmentation time in Mg-based (RT buffer) single cell totoRNAseq protocol | |||
Results: Treatment of RNA for 5min at 94C increased the amplified yield but the size indicates little fragmentation occurred; no library recovery following DSN treatment | |||
[[File:03112013.pdf]] | [[File:03112013.pdf]] |
Latest revision as of 22:16, 14 March 2013
Purpose: Increase fragmentation time in Mg-based (RT buffer) single cell totoRNAseq protocol
Results: Treatment of RNA for 5min at 94C increased the amplified yield but the size indicates little fragmentation occurred; no library recovery following DSN treatment