03112013: Difference between revisions

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Purpose: Increase fragmentation time in Mg-based (RT buffer) single cell totoRNAseq protocol
Results: Treatment of RNA for 5min at 94C increased the amplified yield but the size indicates little fragmentation occurred; no library recovery following DSN treatment
[[File:03112013.pdf]]
[[File:03112013.pdf]]

Latest revision as of 22:16, 14 March 2013

Purpose: Increase fragmentation time in Mg-based (RT buffer) single cell totoRNAseq protocol

Results: Treatment of RNA for 5min at 94C increased the amplified yield but the size indicates little fragmentation occurred; no library recovery following DSN treatment


File:03112013.pdf