EricChu:LabNotesMDA/2013-3-22: Difference between revisions
Jump to navigation
Jump to search
>Ericchu (Created page with "===Quantification of Mouse gDNA MDA in device by qPCR=== * Remainder: conditions of sample 1-16 had high Phi29 concentration and sample 17-24 had normal Phi29 concentration (...") |
>Ericchu No edit summary |
||
(4 intermediate revisions by the same user not shown) | |||
Line 2: | Line 2: | ||
* Remainder: conditions of sample 1-16 had high Phi29 concentration and sample 17-24 had normal Phi29 concentration (see March 20 notes) | * Remainder: conditions of sample 1-16 had high Phi29 concentration and sample 17-24 had normal Phi29 concentration (see March 20 notes) | ||
[[File:03222013qPCR.jpg]] | [[File:03222013qPCR.jpg]] | ||
* qPCR curve of B2a/b standard with samples below | |||
[[File:03212013B2.jpg]] | |||
* qPCR curve of mSINE standard with samples below | |||
[[File:03222013msine.jpg]] | |||
* Images of DNA clusters in different chambers | |||
**Chambers 15-16 used high Phi29 concentration; Chambers 17-18 used low Phi29 concentration | |||
{| {{table}} | |||
| Chamber 15 | |||
|| | |||
| Chamber 16 | |||
|- | |||
| [[File:03222013ch15highPhi29.jpg|300px]] | |||
|| | |||
| [[File:03222013ch16highPhi29.jpg|300px]] | |||
|- | |||
| Chamber 17 | |||
|| | |||
| Chamber 18 | |||
|- | |||
| [[File:03222013ch17lowPhi29.jpg|300px]] | |||
|| | |||
| [[File:03222013ch18lowPhi29.jpg|300px]] | |||
|} |
Latest revision as of 19:51, 26 March 2013
Quantification of Mouse gDNA MDA in device by qPCR[edit]
- Remainder: conditions of sample 1-16 had high Phi29 concentration and sample 17-24 had normal Phi29 concentration (see March 20 notes)
- qPCR curve of B2a/b standard with samples below
- qPCR curve of mSINE standard with samples below
- Images of DNA clusters in different chambers
- Chambers 15-16 used high Phi29 concentration; Chambers 17-18 used low Phi29 concentration
Chamber 15 | Chamber 16 | |
File:03222013ch15highPhi29.jpg | File:03222013ch16highPhi29.jpg | |
Chamber 17 | Chamber 18 | |
File:03222013ch17lowPhi29.jpg | File:03222013ch18lowPhi29.jpg |