EricChu:LabNotesMDA/2013-3-25: Difference between revisions

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=== Using MEF and High Concentration Phi29 in the Vertical Chamber Device ===  
=== Using MEF and High Concentration Phi29 in the Vertical Chamber Device ===  


# pH adjustment for device was tested prior to this experiment. pH of ALS+NS should be = 6.0 to get a neutral pH in the device.
* pH adjustment for device was tested prior to this experiment. pH of ALS+NS should be = 6.0 to get a neutral pH in the device.


# 2 cells were captured in the capture chamber. Cells were lysed in the device.
* 2 cells were captured in the capture chamber. Cells were lysed in the device.


# Air pushing lysed sample to the next area. But visible amount of samples remained in the mixing rings.
* Air pushing lysed sample to the next area. But visible amount of samples remained in the mixing rings.


# Mixing MDA mix and cell contents on device. No observable DNA.
* Mixing MDA mix and cell contents on device. No observable DNA.


# After 5 hours 30C on TE module, clusters of DNA were visible in the high vertical chambers but not the rounded channels. However, the number of clusters (visible DNA) were lower in this experiment.
* After 5 hours 30C on TE module, clusters of DNA were visible in the high vertical chambers but not the rounded channels. However, the number of clusters (visible DNA) were lower in this experiment.

Revision as of 19:34, 26 March 2013

Using MEF and High Concentration Phi29 in the Vertical Chamber Device

  • pH adjustment for device was tested prior to this experiment. pH of ALS+NS should be = 6.0 to get a neutral pH in the device.
  • 2 cells were captured in the capture chamber. Cells were lysed in the device.
  • Air pushing lysed sample to the next area. But visible amount of samples remained in the mixing rings.
  • Mixing MDA mix and cell contents on device. No observable DNA.
  • After 5 hours 30C on TE module, clusters of DNA were visible in the high vertical chambers but not the rounded channels. However, the number of clusters (visible DNA) were lower in this experiment.