Tina:Cardiomyocyte differentiation in H9 ESCs (CDH9): Difference between revisions
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>TinaLo (Created page with "*'''Purpose''': To determine how Metallo Lab's modified cardiomyocyte differentiation protocol works on H9 ESCs. *'''Cells''': Brainbow H9 constructs ==Experiment #1== *Study...") |
>TinaLo m (→Experiment #1) |
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*'''Cells''': Brainbow H9 constructs | *'''Cells''': Brainbow H9 constructs | ||
==Experiment #1== | ==Experiment #1 (CDH9_E1_T1)== | ||
*Study Purpose: Try 3 different induction medium and 2 different cell density during differentiation and see how it works on H9 ESCs directed cardiomyocyte differentiation. | *Study Purpose: Try 3 different induction medium and 2 different cell density during differentiation and see how it works on H9 ESCs directed cardiomyocyte differentiation. | ||
*Date: | *Date: |
Revision as of 01:29, 3 April 2013
- Purpose: To determine how Metallo Lab's modified cardiomyocyte differentiation protocol works on H9 ESCs.
- Cells: Brainbow H9 constructs
Experiment #1 (CDH9_E1_T1)
- Study Purpose: Try 3 different induction medium and 2 different cell density during differentiation and see how it works on H9 ESCs directed cardiomyocyte differentiation.
- Date:
- Cells:
- Protocol used: Christian Metallo Lab protocol.
- Three induction medium used: High Gln induction medium, Low Gln induction medium, DMK induction medium
- Two cell density used: 100%, 80% confluency.
- Experimental design:
12-well plate #1
100% confluency | 1 | 2 | 3 | 4 |
A | High Gln, repeat 1 | High Gln, repeat 2 | High Gln, repeat 3 | High Gln, repeat 4 |
B | Low Gln, repeat 1 | Low Gln, repeat 2 | Low Gln, repeat 3 | Low Gln, repeat 4 |
C | DMK, repeat 1 | DMK, repeat 2 | DMK, repeat 3 | DMK, repeat 4 |
12-well plate #2
80% confluency | 1 | 2 | 3 | 4 |
A | High Gln, repeat 1 | High Gln, repeat 2 | High Gln, repeat 3 | High Gln, repeat 4 |
B | Low Gln, repeat 1 | Low Gln, repeat 2 | Low Gln, repeat 3 | Low Gln, repeat 4 |
C | DMK, repeat 1 | DMK, repeat 2 | DMK, repeat 3 | DMK, repeat 4 |