Daniel:Protocols/EtOHPrecip: Difference between revisions
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=Ethanol Precipitation= | =Ethanol Precipitation= | ||
Purpose: To purifiy DNA. | |||
==Protocol== | |||
1. Add (X=volume sample): | |||
At least 2.5X 100% Ethanol | |||
0.1X 3M NaOAc | |||
1/300X glycoblue (co-precipitate, helps you see the DNA) | |||
2. Incubate for at least 1 hour at -80 C | |||
For smaller fragments it is helpful to incubate overnight | |||
3. Centrifuge on |
Revision as of 23:19, 17 April 2013
Ethanol Precipitation
Purpose: To purifiy DNA.
Protocol
1. Add (X=volume sample): At least 2.5X 100% Ethanol 0.1X 3M NaOAc 1/300X glycoblue (co-precipitate, helps you see the DNA) 2. Incubate for at least 1 hour at -80 C For smaller fragments it is helpful to incubate overnight 3. Centrifuge on