Daniel:Protocols/EtOHPrecip: Difference between revisions

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=Ethanol Precipitation=
=Ethanol Precipitation=
  Purpose:  To purifiy DNA.
==Protocol==
  1. Add (X=volume sample):
    At least 2.5X 100% Ethanol
    0.1X 3M NaOAc
    1/300X glycoblue (co-precipitate, helps you see the DNA)
  2. Incubate for at least 1 hour at -80 C
    For smaller fragments it is helpful to incubate overnight
  3. Centrifuge on

Revision as of 23:19, 17 April 2013

Ethanol Precipitation

 Purpose:  To purifiy DNA.

Protocol

 1. Add (X=volume sample):
   At least 2.5X 100% Ethanol
   0.1X 3M NaOAc
   1/300X glycoblue (co-precipitate, helps you see the DNA)
 2. Incubate for at least 1 hour at -80 C
   For smaller fragments it is helpful to incubate overnight
 3. Centrifuge on