Daniel:Notebook/HiResChrPaint/2013-4-18: Difference between revisions

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=Probe Prep Synthesis ([[Daniel:Notebook/HiResChrPaint/2013-3-26|Started 3/26/13]])=
=Probe Prep Synthesis ([[Daniel:Notebook/HiResChrPaint/2013-3-26|Started 3/26/13]])=
==Ethanol Precipitation ([[Daniel:Notebook/HiResChrPaint/2013-4-17|from 4/17/2013]])==
==Ethanol Precipitation ([[Daniel:Notebook/HiResChrPaint/2013-4-17|from 4/17/2013]])==
  4. Pulled samples from -80C and immediately centrifuged at 14000 rpm for 30 minutes at 4C
  5. Removed supernatant and add 750 uL chilled 75% EtOH
  6. Centriuged 15 minutes at 14000 rpm at 4C
  7. Removed supernatant and let stand in hood for ~30 minutes
  8. Suspended DNA with 6 uL nuclease free water
[[Daniel:Notebook/HiResChrPaint/|Back to Calendar]]

Revision as of 21:11, 18 April 2013

Probe Prep Synthesis (Started 3/26/13)

Ethanol Precipitation (from 4/17/2013)

 4. Pulled samples from -80C and immediately centrifuged at 14000 rpm for 30 minutes at 4C
 5. Removed supernatant and add 750 uL chilled 75% EtOH
 6. Centriuged 15 minutes at 14000 rpm at 4C
 7. Removed supernatant and let stand in hood for ~30 minutes
 8. Suspended DNA with 6 uL nuclease free water

Back to Calendar