Daniel:Notebook/HiResChrPaint/2013-4-18: Difference between revisions
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=Probe Prep Synthesis ([[Daniel:Notebook/HiResChrPaint/2013-3-26|Started 3/26/13]])= | =Probe Prep Synthesis ([[Daniel:Notebook/HiResChrPaint/2013-3-26|Started 3/26/13]])= | ||
==Ethanol Precipitation ([[Daniel:Notebook/HiResChrPaint/2013-4-17|from 4/17/2013]])== | ==Ethanol Precipitation ([[Daniel:Notebook/HiResChrPaint/2013-4-17|from 4/17/2013]])== | ||
4. Pulled samples from -80C and immediately centrifuged at 14000 rpm for 30 minutes at 4C | |||
5. Removed supernatant and add 750 uL chilled 75% EtOH | |||
6. Centriuged 15 minutes at 14000 rpm at 4C | |||
7. Removed supernatant and let stand in hood for ~30 minutes | |||
8. Suspended DNA with 6 uL nuclease free water | |||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] |
Latest revision as of 21:11, 18 April 2013
Probe Prep Synthesis (Started 3/26/13)[edit]
Ethanol Precipitation (from 4/17/2013)[edit]
4. Pulled samples from -80C and immediately centrifuged at 14000 rpm for 30 minutes at 4C 5. Removed supernatant and add 750 uL chilled 75% EtOH 6. Centriuged 15 minutes at 14000 rpm at 4C 7. Removed supernatant and let stand in hood for ~30 minutes 8. Suspended DNA with 6 uL nuclease free water