Blue:RNA-Seq Experiments:04162013: Difference between revisions
Jump to navigation
Jump to search
>B1lake (Created page with "File:04052013.pdf") |
>B1lake No edit summary |
||
Line 1: | Line 1: | ||
Purpose: Test the effects of lysis buffer on RT fragmentation-based single cell RNA-seq protocol | |||
Results: Tested RT buffer with or without Triton-x100 (0.1%, 0.2%), NP-40 (0.1%, 0.2%, 0.5%) or RNase Inhibitor. Used 72C 10 min followed by 94C 5 min. A lot of evaporation occurred and the RNA looked over-fragmented. | |||
[[File:04052013.pdf]] | [[File:04052013.pdf]] |
Latest revision as of 01:01, 19 April 2013
Purpose: Test the effects of lysis buffer on RT fragmentation-based single cell RNA-seq protocol
Results: Tested RT buffer with or without Triton-x100 (0.1%, 0.2%), NP-40 (0.1%, 0.2%, 0.5%) or RNase Inhibitor. Used 72C 10 min followed by 94C 5 min. A lot of evaporation occurred and the RNA looked over-fragmented.