Daniel:Notebook/HiResChrPaint/2013-4-19: Difference between revisions

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[[Image:ProbeQuant_EtOH_041913.jpg|600px]]
[[Image:ProbeQuant_EtOH_041913.jpg|600px]]


==Discussion==
This gel result is meant to be compared against the result from [[Daniel:Notebook/HiResChrPaint/2013-4-10|4/10/13]].  Both methods followed
  the same dye coupling procedure, followed by purification; either column purification (4/10/13) or ethanol precipitation (4/17/13).
  As of yet I have no comment on which method produced better results.


[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]

Revision as of 20:50, 19 April 2013

Probe Prep Synthesis (Started 3/26/13)

TBU Gel (from EtOH Precipitation 4/18/2013)

 1. Preran gel for ~45 minutes at 250V
 2. Ran 2 gels w/5 lanes each (out of 12 well gels)
 3. 5.5 uL clean TBE, 0.5 uL sample, 6 uL 2X Urea buffer (0.5 ladder also)
 4. Precooked samples at 65C for 8 minutes
 5. Loaded all 12 uL into each lane
 6. Ran gels for 20 minutes at 250 V
 7. Stained with 3 uL SYBR gold for 30 minutes
 8. Rinsed, let sit for ~20 minutes 
     *this isn't recommended but I didn't have time to image immediately
 9. Imaged in gel doc

Gel Images

File:ProbeQuant EtOH 041913 V6S2.jpg

File:ProbeQuant EtOH 041913.jpg

Discussion

This gel result is meant to be compared against the result from 4/10/13.  Both methods followed
 the same dye coupling procedure, followed by purification; either column purification (4/10/13) or ethanol precipitation (4/17/13).
 As of yet I have no comment on which method produced better results.

Back to Calendar