Daniel:Notebook/HiResChrPaint/2013-4-29: Difference between revisions
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>Djacobse (Created page with "=Probe Prep Test (Started 4/22/2013)= Back to calendar =TBE Gel= Purpose: As per [[Daniel:Not...") |
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2. Ran gel for | 2. Ran gel for 25 minutes at 200 V | ||
3. Stained with 3 uL SYBR gold for 5 min | 3. Stained with 3 uL SYBR gold for 5 min |
Revision as of 21:54, 29 April 2013
Probe Prep Test (Started 4/22/2013)
TBE Gel
Purpose: As per 4/27/2013 results, I need to better understand the lambda exonuclease digestion. To this end I'm going to play around with the parameters of the reaction. To do so I need several samples to work with. I am testing/identifying samples right now that will suit my purposes.
Protocol
1. Prepared samples (1 uL sample, 5 uL TBE, 2 uL 6x dye) and loaded (7 uL) according to table
Lane | 2 | 3 | 4 | 5 | 6 |
Sample | Ladder | V4S2 | V6S2 | V6S3 | V6S3 Digest |
2. Ran gel for 25 minutes at 200 V 3. Stained with 3 uL SYBR gold for 5 min