Daniel:Notebook/HiResChrPaint/2013-4-1: Difference between revisions
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===Gel Image=== | |||
[[Image:DpnDigest_040113.png|600px]] |
Latest revision as of 00:14, 30 April 2013
Probe Prep (Started 4/26/13)[edit]
DpnII Digestion[edit]
Purpose: Trying to erase strange black marks from previous gels
1. Set up reactions according to table
Probe | ng/uL | uL | 10x Buffer | DpnII | H2O | Total |
V4S1 | 41.6 | 30 | 5 | 5 | 10 | 50 |
V4S2 | 159.8 | 30 | 5 | 5 | 10 | 50 |
V4S3 | 73.1 | 30 | 5 | 5 | 10 | 50 |
V4S4 | 47.8 | 30 | 5 | 5 | 10 | 50 |
V6S1 | 84.4 | 30 | 5 | 5 | 10 | 50 |
V6S2 | 111.9 | 30 | 5 | 5 | 10 | 50 |
V6S3 | 131.4 | 30 | 5 | 5 | 10 | 50 |
V6S4 | 106.9 | 30 | 5 | 5 | 10 | 50 |
2. Incubate at 37 C for 1 hr; 20 minute heat shock at 65C 3. Column purification, elute with 30 uL 4. Measure concentration in Nanodrop
Nanodrop Results[edit]
Probe | ng/uL | uL | ng |
V4S1 | 21.9 | 30 | 657 |
V4S2 | 120.6 | 30 | 3618 |
V4S3 | 53.6 | 30 | 1608 |
V4S4 | 25 | 30 | 750 |
V6S1 | 61.2 | 30 | 1836 |
V6S2 | 82 | 30 | 2460 |
V6S3 | 105.3 | 30 | 3159 |
V6S4 | 80.6 | 30 | 2418 |