Hosuk:LabNotes/2013-5-2: Difference between revisions
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>Hosuki78 (Created page with "====Result - RT check==== *based on Debugging plan (05/01) *+RTase sample showed very strong Cy5 signal which is supposed cDNA *comparing to -RTas...") |
>Hosuki78 No edit summary |
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*not many spots were observed, which spots were outside of cells. | *not many spots were observed, which spots were outside of cells. | ||
*'''+RTase''' | |||
{| {{table}} | {| {{table}} | ||
| S2:+RTase, Brighfield, 20x | | S2:+RTase, Brighfield, 20x | ||
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|[[File:Composite_+RTase_fig07-08.jpg|300px]] | |[[File:Composite_+RTase_fig07-08.jpg|300px]] | ||
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*'''-RTase''' | |||
{| {{table}} | |||
| S3:-RTase, Brighfield, 20x | |||
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| S3:-RTase, Cy5, 20x | |||
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| S3:-RTase, Merged | |||
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|([[Media:RTCheckNoRTase_fig08_20xobj_pos05_BF_exp0.2-EM00.tif|Original]]) | |||
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|([[Media:RTCheckNoRTase_fig07_20xobj_pos04_Cy5_exp0.2-EM40.tif|Original]]) | |||
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|- | |||
|[[File:RTCheckNoRTase_fig08_20xobj_pos05_BF_exp0.2-EM00.jpg|300px]] | |||
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|[[File:RTCheckNoRTase_fig07_20xobj_pos04_Cy5_exp0.2-EM40.jpg|300px]] | |||
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|[[File:Composite_NoRTase_fig07-08.jpg|300px]] | |||
|} | |} |
Revision as of 22:34, 2 May 2013
Result - RT check
- based on Debugging plan (05/01)
- +RTase sample showed very strong Cy5 signal which is supposed cDNA
- comparing to -RTase result, the bright singal over the cell seemed they were cDNAs
- Most of signal came from cell structure.
- However I don't know what are the large spots. Are they also cDNAs?
- -RTase sample has very low intensity of signals in the cell, and
- not many spots were observed, which spots were outside of cells.
- +RTase
S2:+RTase, Brighfield, 20x | S2:+RTase, Cy5, 20x | S2:+RTase, Merged | |||
(Original) | (Original) | ||||
File:RTCheck +RTase fig08 20xobj pos04 BF exp0.2-EM00.jpg | File:RTCheck +RTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg | File:Composite +RTase fig07-08.jpg |
- -RTase
S3:-RTase, Brighfield, 20x | S3:-RTase, Cy5, 20x | S3:-RTase, Merged | |||
(Original) | (Original) | ||||
File:RTCheckNoRTase fig08 20xobj pos05 BF exp0.2-EM00.jpg | File:RTCheckNoRTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg | File:Composite NoRTase fig07-08.jpg |