Daniel:Notebook/HiResChrPaint/2013-5-8: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
No edit summary
>Djacobse
No edit summary
Line 92: Line 92:


|}
|}
2. Heat shock 10 minutes at 75 C
3. Run gel for 24 minutes at 250V
4. Stain with 3 uL SYBR gold for 20 minutes
===Gel Results===

Revision as of 22:29, 8 May 2013

Probe Prep Test (Started 4/22/2013)

Back to Calendar

Column Purification vs. Ethanol Precipitation

Ethanol Precipitation (continued from 5/6/2013)

5. Centrifuged 30 min at 10000 rpm at 4C
6. Removed supernatant and added 750 uL chilled EtOH
7. Centrifuged 15 min at 10000 rpm at 4C
8. Removed supernatant and dried in hood
9. Resuspended in 10 uL nf H20

TBU Gel comparison

Protocol 2

1. Gel lanes
Sample 0.5 Ladder 1 uL Ladder Original Column (1uL) Column (2uL) EtOH (1 uL) A EtOH (2uL) A EtOH (1 uL) B EtOH (2uL) B
uL sample 0.5 1 0.5 0.5 0.5 0.5 0.5 0.5 0.5
uL TBE 9.5 9 9.5 9.5 9.5 9.5 9.5 9.5 9.5
uL 2X buffer 10 10 10 10 10 10 10 10 10
Loaded 4 4 4 4 4 4 4 4 4
Dilution 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1
2. Heat shock 10 minutes at 75 C
3. Run gel for 24 minutes at 250V
4. Stain with 3 uL SYBR gold for 20 minutes

Gel Results