Matt:LabNotes/2013-5-17: Difference between revisions

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Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-16
Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-16


===PAGE-Size Selection of 0 gap probes===
===PAGE-Size Selection of 20 gap probes===


*Noi said that the band on the previous TBU gel looked relatively faint and recommended putting 3ug of DNA per well instead of the usual 2ug to increase yield
*Noi said that the band on the previous TBU gel looked relatively faint and recommended putting 3ug of DNA per well instead of the usual 2ug to increase yield
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| align="center" style="background:#f0f0f0;"|'''3X Volume'''
| align="center" style="background:#f0f0f0;"|'''3X Volume'''
|-
|-
| Enzyme digested probes (~10ug/180ul||60||180
| Enzyme digested probes (~10ug/180ul)||60||180
|-
|-
| TBE-Urea Buffer 2X||60||180
| TBE-Urea Buffer 2X||60||180

Revision as of 20:50, 17 May 2013

Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-16

PAGE-Size Selection of 20 gap probes

  • Noi said that the band on the previous TBU gel looked relatively faint and recommended putting 3ug of DNA per well instead of the usual 2ug to increase yield
  • I will run 3 gels each with 60ul (~3.4 ug of DNA each)

Sample

Components 1X Volume 3X Volume
Enzyme digested probes (~10ug/180ul) 60 180
TBE-Urea Buffer 2X 60 180
Water 0 0
Total 120 360

3 Low Mass DNA Ladders

Components 2X Volume
Low Mass DNA ladder (1 ug/ul) 3
TBE-Urea Buffer 2X 15
H2O 12
Total 30