Matt:LabNotes/2013-5-17: Difference between revisions
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>Mzcai (Created page with "Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-16 ===PAGE-Size Selection of 0 gap probes=== *Noi said that the band on the previous TBU ...") |
>Mzcai mNo edit summary |
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Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-16 | Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-16 | ||
===PAGE-Size Selection of | ===PAGE-Size Selection of 20 gap probes=== | ||
*Noi said that the band on the previous TBU gel looked relatively faint and recommended putting 3ug of DNA per well instead of the usual 2ug to increase yield | *Noi said that the band on the previous TBU gel looked relatively faint and recommended putting 3ug of DNA per well instead of the usual 2ug to increase yield | ||
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| align="center" style="background:#f0f0f0;"|'''3X Volume''' | | align="center" style="background:#f0f0f0;"|'''3X Volume''' | ||
|- | |- | ||
| Enzyme digested probes (~10ug/180ul||60||180 | | Enzyme digested probes (~10ug/180ul)||60||180 | ||
|- | |- | ||
| TBE-Urea Buffer 2X||60||180 | | TBE-Urea Buffer 2X||60||180 |
Revision as of 20:50, 17 May 2013
Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-16
PAGE-Size Selection of 20 gap probes
- Noi said that the band on the previous TBU gel looked relatively faint and recommended putting 3ug of DNA per well instead of the usual 2ug to increase yield
- I will run 3 gels each with 60ul (~3.4 ug of DNA each)
Sample
Components | 1X Volume | 3X Volume |
Enzyme digested probes (~10ug/180ul) | 60 | 180 |
TBE-Urea Buffer 2X | 60 | 180 |
Water | 0 | 0 |
Total | 120 | 360 |
3 Low Mass DNA Ladders
Components | 2X Volume |
Low Mass DNA ladder (1 ug/ul) | 3 |
TBE-Urea Buffer 2X | 15 |
H2O | 12 |
Total | 30 |