Matt:LabNotes/2013-5-17: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''3X Volume''' | | align="center" style="background:#f0f0f0;"|'''3X Volume''' | ||
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| Enzyme digested probes (~10ug/180ul)|| | | Enzyme digested probes (~10ug/180ul)||55||165 | ||
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| TBE-Urea Buffer 2X|| | | TBE-Urea Buffer 2X||55||165 | ||
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| Water||0||0 | | Water||0||0 | ||
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| Total|| | | Total||110||330 | ||
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Revision as of 21:05, 17 May 2013
Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-16
PAGE-Size Selection of 20 gap probes
- Noi said that the band on the previous TBU gel looked relatively faint and recommended putting 3ug of DNA per well instead of the usual 2ug to increase yield
- I will run 3 gels each with 60ul (~3.4 ug of DNA each)
Sample
Components | 1X Volume | 3X Volume |
Enzyme digested probes (~10ug/180ul) | 55 | 165 |
TBE-Urea Buffer 2X | 55 | 165 |
Water | 0 | 0 |
Total | 110 | 330 |
3 Low Mass DNA Ladders
Components | 3X Volume |
Low Mass DNA ladder (1 ug/ul) | 3 |
TBE-Urea Buffer 2X | 15 |
H2O | 12 |
Total | 30 |