EricChu:LabNotesMDA/2013-5-21: Difference between revisions

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== Post-CoRE Library Construction and PCR Verification (accompany to Noi/NOTES/2013-5-22) ==
== Post-CoRE Library Construction and PCR Verification (accompany to Noi/NOTES/2013-5-22) ==
* 16 samples prepared by CoRE was given to
* 16 samples prepared by CoRE was given to

Revision as of 23:40, 22 May 2013

MDA Samples Collection from MDA Device v7

  • Some valve lines became empty overnight
  • All samples were pushed down from the chambers by TE buffer to reduce mixing
  • Center 8 samples were collected first
  • Bubbles were generated from empty valve lines during sample collection. Video was taken when bubbles pushed the samples away from chamber 23, and that caused mixing.
  • All chambers were collected in about 6ul volume.

qPCR Verification of MDA

  • qPCR result of the 2nd single MEF cell experiments is similar to the 1st experiment.
  • Out of 24 samples,
  • A total of 8 samples yielded over 1ng product.
  • A total of 8 samples yielded 500pg-1ng product.
  • 3 samples yielded 100pg-500pg and 5 samples yielded less than 100pg.
  • All 8 samples of low yield (<500pg) were coming from the chambers 18-24

File:05242013qPCRa.jpg

chamber total (pg)
1 937.82126
2 1660.2249
3 1587.6281
4 1696.0932
5 500.50544
6 548.40513
7 1025.5768
8 2111.1235
9 1805.3481
10 855.75854
11 1264.0812
12 715.56902
13 755.17198
14 762.9601
15 453.42019
16 649.84148
17 1271.5977
18 35.038787
19 11.20207
20 19.804252
21 127.08806
22 20.274461
23 110.39759
24 95.640833


Post-CoRE Library Construction and PCR Verification (accompany to Noi/NOTES/2013-5-22)

  • 16 samples prepared by CoRE was given to