Matt:LabNotes/2013-5-29: Difference between revisions

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>Mzcai
(Created page with "===Page Size Selection===")
 
>Mzcai
Line 1: Line 1:
===Page Size Selection===
===Page Size Selection===
*Noi prepared some 0 gap and 20 gap probes
  *20 gap: 12.6ug
  *0 gap:
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Components'''
| align="center" style="background:#f0f0f0;"|'''1X Volume'''
| align="center" style="background:#f0f0f0;"|'''4X Volume'''
|-
| Enzyme digested probes (~12.6ug/400ul)||105||420
|-
| TBE-Urea Buffer 2X||105||420
|-
| H2O||0||0
|-
| Total||210||840
|-
|
|}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Components'''
| align="center" style="background:#f0f0f0;"|'''1X Volume'''
| align="center" style="background:#f0f0f0;"|'''4X Volume'''
|-
| 10-bp DNA ladder (1 ug/ul)||1||4
|-
| TBE-Urea Buffer 2X||5||20
|-
| H2O||4||16
|-
| total||10||40
|-
|
|}

Revision as of 20:57, 29 May 2013

Page Size Selection

  • Noi prepared some 0 gap and 20 gap probes
 *20 gap: 12.6ug
 *0 gap: 
Components 1X Volume 4X Volume
Enzyme digested probes (~12.6ug/400ul) 105 420
TBE-Urea Buffer 2X 105 420
H2O 0 0
Total 210 840
Components 1X Volume 4X Volume
10-bp DNA ladder (1 ug/ul) 1 4
TBE-Urea Buffer 2X 5 20
H2O 4 16
total 10 40