Matt:LabNotes/2013-5-29: Difference between revisions
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>Mzcai (Created page with "===Page Size Selection===") |
>Mzcai |
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===Page Size Selection=== | ===Page Size Selection=== | ||
*Noi prepared some 0 gap and 20 gap probes | |||
*20 gap: 12.6ug | |||
*0 gap: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Components''' | |||
| align="center" style="background:#f0f0f0;"|'''1X Volume''' | |||
| align="center" style="background:#f0f0f0;"|'''4X Volume''' | |||
|- | |||
| Enzyme digested probes (~12.6ug/400ul)||105||420 | |||
|- | |||
| TBE-Urea Buffer 2X||105||420 | |||
|- | |||
| H2O||0||0 | |||
|- | |||
| Total||210||840 | |||
|- | |||
| | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Components''' | |||
| align="center" style="background:#f0f0f0;"|'''1X Volume''' | |||
| align="center" style="background:#f0f0f0;"|'''4X Volume''' | |||
|- | |||
| 10-bp DNA ladder (1 ug/ul)||1||4 | |||
|- | |||
| TBE-Urea Buffer 2X||5||20 | |||
|- | |||
| H2O||4||16 | |||
|- | |||
| total||10||40 | |||
|- | |||
| | |||
|} |
Revision as of 20:57, 29 May 2013
Page Size Selection
- Noi prepared some 0 gap and 20 gap probes
*20 gap: 12.6ug *0 gap:
Components | 1X Volume | 4X Volume |
Enzyme digested probes (~12.6ug/400ul) | 105 | 420 |
TBE-Urea Buffer 2X | 105 | 420 |
H2O | 0 | 0 |
Total | 210 | 840 |
Components | 1X Volume | 4X Volume |
10-bp DNA ladder (1 ug/ul) | 1 | 4 |
TBE-Urea Buffer 2X | 5 | 20 |
H2O | 4 | 16 |
total | 10 | 40 |