Noi/NOTES/2013-5-30: Difference between revisions
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** Volume 80ul in TE buffer. Total amount 3.21ug. | ** Volume 80ul in TE buffer. Total amount 3.21ug. | ||
* Probe prep plan | * Probe prep plan | ||
** Last time of Matt's experiment on Expansion PCR of oligo from CustomArray, we need to used template ~100x more than probe synthesize from Agilent. This time I will test for expansion PCR using different amount of oligo template (concentration in expansion PCR 20nM and 100nM) and | ** Last time of Matt's experiment on Expansion PCR of oligo from CustomArray, we need to used template ~100x more than probe synthesize from Agilent. This time I will test for expansion PCR using different amount of oligo template (concentration in expansion PCR 20nM and 100nM) and useDMR330k oligos (S1) from Agilent as PC. | ||
** Aliquot 20ul oligo from original tube and dilute to 500nM (add 12.45ul to 20ul of 811.3nM oligo) | ** Aliquot 20ul oligo from original tube and dilute to 500nM (add 12.45ul to 20ul of 811.3nM oligo) |
Revision as of 18:51, 30 May 2013
DNA methylation assay for Blueprint project
- 2013-05-30: Received oligos from CustomArray
- Oligo information
- Design: BSPP_Design-5-17-2013 --> I would name BSPP_Blueprint for future experiment
- Length 150 mer (MW = 150 * 330Da/bp = 49,500 Da or g/mole
- Cocn. 40.16ng/ul = 811.3 nM (40.16ng/ul/ 49,500 g/mole)
- Volume 80ul in TE buffer. Total amount 3.21ug.
- Probe prep plan
- Last time of Matt's experiment on Expansion PCR of oligo from CustomArray, we need to used template ~100x more than probe synthesize from Agilent. This time I will test for expansion PCR using different amount of oligo template (concentration in expansion PCR 20nM and 100nM) and useDMR330k oligos (S1) from Agilent as PC.
- Aliquot 20ul oligo from original tube and dilute to 500nM (add 12.45ul to 20ul of 811.3nM oligo)