Matt:LabNotes/2013-5-31: Difference between revisions

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===Probe Capture===
===Probe Capture===
*Use the ~18 pmole of probes from [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22] and [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-29] to produce maximum number of circularized probes with U's incorporated for Ho Suk's experiments
*Use the ~37.4 pmole of probes from [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22] and [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-29] to produce maximum number of circularized probes with U's incorporated for Ho Suk's experiments
*Use gDNA as template/target
*Use gDNA as template/target
*Use a probe:target ratio of 100:1


====Probe:Target====
====100:1 Probe:Target Calculations====
{| {{table}}
| Total Probes||3.74E-11||mole
|-
| Probes||2.88491E-15||mole (Total Probe/12,964 20 gap probes)
|-
| Target||2.88491E-17||mole (Probes/100)
|-
| Human gDNA MW||1.95E+12||g/mol (3x10^9 bp x 650 Da/bp + 157.9 Da)
|-
| Human gDNA mass||5.62558E-05||g (Target x Human gDNA MW)
|-
|
|}

Revision as of 20:39, 31 May 2013

Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22

Probe Capture

  • Use the ~37.4 pmole of probes from [1] and [2] to produce maximum number of circularized probes with U's incorporated for Ho Suk's experiments
  • Use gDNA as template/target
  • Use a probe:target ratio of 100:1

100:1 Probe:Target Calculations

Total Probes 3.74E-11 mole
Probes 2.88491E-15 mole (Total Probe/12,964 20 gap probes)
Target 2.88491E-17 mole (Probes/100)
Human gDNA MW 1.95E+12 g/mol (3x10^9 bp x 650 Da/bp + 157.9 Da)
Human gDNA mass 5.62558E-05 g (Target x Human gDNA MW)