Daniel:Notebook/HiResChrPaint/2013-6-2: Difference between revisions

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  2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C
  2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C
3. [[Daniel:Protocols/Qiaquick_Column|Column Purification]], eluted with 30 uL EB
4. Stored at 4C; Gel running [[Daniel:Notebook/HiResChrPaint/2013-6-3|tomorrow]]
==Nanodrop Results (from 6/3/2013)==

Revision as of 23:12, 3 June 2013

Probe Prep 3 (Started 5/29/2013)

DpnII Digestion Test

Only running 1/4 of DNA I have for each sample to compare DpnII reaction yield using aadUTP DNA and normal DNA.
1. Set up 
Sample uL sample uL Dpn II (50 U/uL) uL 10x buffer uL H20 total
alpha 4-A 35 2 5 8 50
alpha 6-A 35 2 5 8 50
beta 4-A 35 2 5 8 50
beta 6-A 35 2 5 8 50
2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C
3. Column Purification, eluted with 30 uL EB
4. Stored at 4C; Gel running tomorrow

Nanodrop Results (from 6/3/2013)