Daniel:Notebook/HiResChrPaint/2013-6-2: Difference between revisions
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>Djacobse (Created page with "=Probe Prep 3 (Started 5/29/2013)= ==DpnII Digestion Test== Only running 1/4 of DNA I have for each sample to compare DpnII reac...") |
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2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C | 2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C | ||
3. [[Daniel:Protocols/Qiaquick_Column|Column Purification]], eluted with 30 uL EB | |||
4. Stored at 4C; Gel running [[Daniel:Notebook/HiResChrPaint/2013-6-3|tomorrow]] | |||
==Nanodrop Results (from 6/3/2013)== |
Revision as of 23:12, 3 June 2013
Probe Prep 3 (Started 5/29/2013)
DpnII Digestion Test
Only running 1/4 of DNA I have for each sample to compare DpnII reaction yield using aadUTP DNA and normal DNA.
1. Set up
Sample | uL sample | uL Dpn II (50 U/uL) | uL 10x buffer | uL H20 | total |
alpha 4-A | 35 | 2 | 5 | 8 | 50 |
alpha 6-A | 35 | 2 | 5 | 8 | 50 |
beta 4-A | 35 | 2 | 5 | 8 | 50 |
beta 6-A | 35 | 2 | 5 | 8 | 50 |
2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C 3. Column Purification, eluted with 30 uL EB 4. Stored at 4C; Gel running tomorrow