Daniel:Notebook/HiResChrPaint/2013-6-2: Difference between revisions

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=Probe Prep 3 ([[Daniel:Notebook/HiResChrPaint/2013-5-29|Started 5/29/2013]])=
=Probe Prep 3 ([[Daniel:Notebook/HiResChrPaint/2013-5-29|Started 5/29/2013]])=
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]


==DpnII Digestion Test==
==DpnII Digestion Test==

Revision as of 21:15, 4 June 2013

Probe Prep 3 (Started 5/29/2013)

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DpnII Digestion Test

Only running 1/4 of DNA I have for each sample to compare DpnII reaction yield using aadUTP DNA and normal DNA.
1. Set up 
Sample uL sample uL Dpn II (50 U/uL) uL 10x buffer uL H20 total
alpha 4-A 35 2 5 8 50
alpha 6-A 35 2 5 8 50
beta 4-A 35 2 5 8 50
beta 6-A 35 2 5 8 50
2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C
3. Column Purification, eluted with 30 uL EB
4. Stored at 4C; Gel running tomorrow

Nanodrop Results (from 6/3/2013)