Daniel:Notebook/HiResChrPaint/2013-6-2: Difference between revisions
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=Probe Prep 3 ([[Daniel:Notebook/HiResChrPaint/2013-5-29|Started 5/29/2013]])= | =Probe Prep 3 ([[Daniel:Notebook/HiResChrPaint/2013-5-29|Started 5/29/2013]])= | ||
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]] | |||
==DpnII Digestion Test== | ==DpnII Digestion Test== | ||
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4. Stored at 4C; Gel running [[Daniel:Notebook/HiResChrPaint/2013-6-3|tomorrow]] | 4. Stored at 4C; Gel running [[Daniel:Notebook/HiResChrPaint/2013-6-3|tomorrow]] | ||
==Nanodrop Results (from 6/ | ==Nanodrop Results (from 6/4/2013)== | ||
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> | |||
|- style="font-size:12pt;font-weight:bold" align="center" valign="bottom" | |||
| width="65" height="15" | Sample | |||
| width="65" | ng/uL | |||
| width="65" | ug in 30 uL | |||
| width="65" | Yield (%) | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
|style="font-weight:bold" height="15" | alpha-4A | |||
| align="center" | 121.5 | |||
| align="center" | 3.6 | |||
| align="center" | 79.9 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
|style="font-weight:bold" height="15" | alpha-6A | |||
| align="center" | 136.7 | |||
| align="center" | 4.1 | |||
| align="center" | 78.1 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
|style="font-weight:bold" height="15" | beta-4A | |||
| align="center" | 126.4 | |||
| align="center" | 3.8 | |||
| align="center" | 73.7 | |||
|- style="font-size:12pt" align="center" valign="bottom" | |||
|style="font-weight:bold" height="15" | beta-6A | |||
| align="center" | 129.7 | |||
| align="center" | 3.9 | |||
| align="center" | 75.7 | |||
|} |
Latest revision as of 21:18, 4 June 2013
Probe Prep 3 (Started 5/29/2013)[edit]
DpnII Digestion Test[edit]
Only running 1/4 of DNA I have for each sample to compare DpnII reaction yield using aadUTP DNA and normal DNA.
1. Set up
Sample | uL sample | uL Dpn II (50 U/uL) | uL 10x buffer | uL H20 | total |
alpha 4-A | 35 | 2 | 5 | 8 | 50 |
alpha 6-A | 35 | 2 | 5 | 8 | 50 |
beta 4-A | 35 | 2 | 5 | 8 | 50 |
beta 6-A | 35 | 2 | 5 | 8 | 50 |
2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C 3. Column Purification, eluted with 30 uL EB 4. Stored at 4C; Gel running tomorrow
Nanodrop Results (from 6/4/2013)[edit]
Sample | ng/uL | ug in 30 uL | Yield (%) |
alpha-4A | 121.5 | 3.6 | 79.9 |
alpha-6A | 136.7 | 4.1 | 78.1 |
beta-4A | 126.4 | 3.8 | 73.7 |
beta-6A | 129.7 | 3.9 | 75.7 |