Daniel:Notebook/HiResChrPaint/2013-6-2: Difference between revisions

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  4. Stored at 4C; Gel running [[Daniel:Notebook/HiResChrPaint/2013-6-3|tomorrow]]
  4. Stored at 4C; Gel running [[Daniel:Notebook/HiResChrPaint/2013-6-3|tomorrow]]


==Nanodrop Results (from 6/3/2013)==
==Nanodrop Results (from 6/4/2013)==
 
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt;font-weight:bold" align="center" valign="bottom"
| width="65" height="15" | Sample
| width="65" | ng/uL
| width="65" | ug in 30 uL
| width="65" | Yield (%)
 
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | alpha-4A
| align="center" | 121.5
| align="center" | 3.6
| align="center" | 79.9
 
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | alpha-6A
| align="center" | 136.7
| align="center" | 4.1
| align="center" | 78.1
 
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | beta-4A
| align="center" | 126.4
| align="center" | 3.8
| align="center" | 73.7
 
|- style="font-size:12pt" align="center" valign="bottom"
|style="font-weight:bold" height="15" | beta-6A
| align="center" | 129.7
| align="center" | 3.9
| align="center" | 75.7
 
|}

Latest revision as of 21:18, 4 June 2013

Probe Prep 3 (Started 5/29/2013)[edit]

Back to Calendar

DpnII Digestion Test[edit]

Only running 1/4 of DNA I have for each sample to compare DpnII reaction yield using aadUTP DNA and normal DNA.
1. Set up 
Sample uL sample uL Dpn II (50 U/uL) uL 10x buffer uL H20 total
alpha 4-A 35 2 5 8 50
alpha 6-A 35 2 5 8 50
beta 4-A 35 2 5 8 50
beta 6-A 35 2 5 8 50
2. Incubate in thermocycler at 37C for 2 hours; heat shock 20 minutes at 65 C
3. Column Purification, eluted with 30 uL EB
4. Stored at 4C; Gel running tomorrow

Nanodrop Results (from 6/4/2013)[edit]

Sample ng/uL ug in 30 uL Yield (%)
alpha-4A 121.5 3.6 79.9
alpha-6A 136.7 4.1 78.1
beta-4A 126.4 3.8 73.7
beta-6A 129.7 3.9 75.7