Hosuk:LabNotes/2013-6-12: Difference between revisions

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>Hosuki78
(Created page with "====Day 3==== *Jay finished last step, washing RCA and binding Cy3 probe, and image *Rolonies are pretty well generated inside cells, *and the signal is very strong, so there ...")
 
>Hosuki78
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*Even though there were some of debris, they could be come from fixing step.
*Even though there were some of debris, they could be come from fixing step.
*No RCA signal were in -RT sample
*No RCA signal were in -RT sample
=====20x, +RT vs. -RT  (1 wash)
{| {{table}}
|Raw images : [[Media:+RT_1wash_ch00.tif|+RT]], [[Media:-RT_1wash_ch00.tif|-RT]]
|-
|[[File:20x_1wash.png|500px]]
|}

Revision as of 21:24, 12 June 2013

Day 3

  • Jay finished last step, washing RCA and binding Cy3 probe, and image
  • Rolonies are pretty well generated inside cells,
  • and the signal is very strong, so there were almost no background
  • Even though there were some of debris, they could be come from fixing step.
  • No RCA signal were in -RT sample

=====20x, +RT vs. -RT (1 wash)

Raw images : +RT, -RT
File:20x 1wash.png