Hosuk:LabNotes/2013-6-12: Difference between revisions
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>Hosuki78 (Created page with "====Day 3==== *Jay finished last step, washing RCA and binding Cy3 probe, and image *Rolonies are pretty well generated inside cells, *and the signal is very strong, so there ...") |
>Hosuki78 No edit summary |
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*Even though there were some of debris, they could be come from fixing step. | *Even though there were some of debris, they could be come from fixing step. | ||
*No RCA signal were in -RT sample | *No RCA signal were in -RT sample | ||
=====20x, +RT vs. -RT (1 wash) | |||
{| {{table}} | |||
|Raw images : [[Media:+RT_1wash_ch00.tif|+RT]], [[Media:-RT_1wash_ch00.tif|-RT]] | |||
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|[[File:20x_1wash.png|500px]] | |||
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Revision as of 21:24, 12 June 2013
Day 3
- Jay finished last step, washing RCA and binding Cy3 probe, and image
- Rolonies are pretty well generated inside cells,
- and the signal is very strong, so there were almost no background
- Even though there were some of debris, they could be come from fixing step.
- No RCA signal were in -RT sample
=====20x, +RT vs. -RT (1 wash)
Raw images : +RT, -RT |
File:20x 1wash.png |