Hosuk:LabNotes/2013-6-12: Difference between revisions

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=====20x, +RT vs. -RT (1 wash)=====
=====20x, +RT vs. -RT (1 wash)=====
{| {{table}}
{| {{table}}
|Raw images : [[Media:+RT_1wash_ch00.tif|+RT]], [[Media:-RT_1wash_ch00.tif|-RT]]
|Raw images : [[Media:+RT_1wash_ch00.tif|+RT]], [[Media:-RT_1wash_ch00.tif|-RT]]
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=====20x, +RT, NA=0.4 vs. 0.8 (2 wash)=====
=====20x, +RT, NA=0.4 vs. 0.8 (2 wash)=====
{| {{table}}
{| {{table}}
|Raw images : [[Media:20x_0.4NA_2wash_same setting_ch00.tif|NA=0.4]], [[Media:20x_0.8NA_2wash_same setting_ch00.tif|NA=0.8]]
|Raw images : [[Media:20x_0.4NA_2wash_same setting_ch00.tif|NA=0.4]], [[Media:20x_0.8NA_2wash_same setting_ch00.tif|NA=0.8]]
|-
|-
|[[File:20x_2wash_0.4NA_0.8NA.png|500px]]
|[[File:20x_2wash_0.4NA_0.8NA.png|500px]]
|}
=====40x, +RT, NA=0.8, Cy3 vs. GFP channel (2 wash)=====
{| {{table}}
|Raw images : [[Media:40x_0.8NA_2wash_ch00.tif|Cy3]], [[Media:40x_0.8NA_2wash_GFPcontrol_ch00.tif|GFP]]
|-
|[[File:40x_2wash_0.8NA_Cy3_GFP.png|500px]]
|}
=====63x, +RT, NA=1.2, Cy3 (2 wash)=====
{| {{table}}
|Raw images : [[Media:63x_2wash_1.2NA_Cy3.tif|63x]]
|-
|[[File:63x_1.2NA_2wash_50msec_100EMgain_ch00.png|500px]]
|}
|}

Revision as of 21:32, 12 June 2013

Day 3

  • Jay finished last step, washing RCA and binding Cy3 probe, and image
  • Rolonies are pretty well generated inside cells,
  • and the signal is very strong, so there were almost no background
  • Even though there were some of debris, they could be come from fixing step.
  • No RCA signal were in -RT sample


20x, +RT vs. -RT (1 wash)
Raw images : +RT, -RT
File:20x 1wash.png


20x, +RT, NA=0.4 vs. 0.8 (2 wash)
Raw images : NA=0.4, NA=0.8
File:20x 2wash 0.4NA 0.8NA.png


40x, +RT, NA=0.8, Cy3 vs. GFP channel (2 wash)
Raw images : Cy3, GFP
File:40x 2wash 0.8NA Cy3 GFP.png


63x, +RT, NA=1.2, Cy3 (2 wash)
Raw images : 63x
File:63x 1.2NA 2wash 50msec 100EMgain ch00.png