Hosuk:LabNotes/2013-6-12: Difference between revisions
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>Hosuki78 No edit summary |
>Hosuki78 No edit summary |
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=====20x, +RT vs. -RT | =====20x, +RT vs. -RT (1 wash)===== | ||
{| {{table}} | {| {{table}} | ||
|Raw images : [[Media:+RT_1wash_ch00.tif|+RT]], [[Media:-RT_1wash_ch00.tif|-RT]] | |Raw images : [[Media:+RT_1wash_ch00.tif|+RT]], [[Media:-RT_1wash_ch00.tif|-RT]] | ||
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=====20x, +RT, NA=0.4 vs. 0.8 | =====20x, +RT, NA=0.4 vs. 0.8 (2 wash)===== | ||
{| {{table}} | {| {{table}} | ||
|Raw images : [[Media:20x_0.4NA_2wash_same setting_ch00.tif|NA=0.4]], [[Media:20x_0.8NA_2wash_same setting_ch00.tif|NA=0.8]] | |Raw images : [[Media:20x_0.4NA_2wash_same setting_ch00.tif|NA=0.4]], [[Media:20x_0.8NA_2wash_same setting_ch00.tif|NA=0.8]] | ||
|- | |- | ||
|[[File:20x_2wash_0.4NA_0.8NA.png|500px]] | |[[File:20x_2wash_0.4NA_0.8NA.png|500px]] | ||
|} | |||
=====40x, +RT, NA=0.8, Cy3 vs. GFP channel (2 wash)===== | |||
{| {{table}} | |||
|Raw images : [[Media:40x_0.8NA_2wash_ch00.tif|Cy3]], [[Media:40x_0.8NA_2wash_GFPcontrol_ch00.tif|GFP]] | |||
|- | |||
|[[File:40x_2wash_0.8NA_Cy3_GFP.png|500px]] | |||
|} | |||
=====63x, +RT, NA=1.2, Cy3 (2 wash)===== | |||
{| {{table}} | |||
|Raw images : [[Media:63x_2wash_1.2NA_Cy3.tif|63x]] | |||
|- | |||
|[[File:63x_1.2NA_2wash_50msec_100EMgain_ch00.png|500px]] | |||
|} | |} |
Revision as of 21:32, 12 June 2013
Day 3
- Jay finished last step, washing RCA and binding Cy3 probe, and image
- Rolonies are pretty well generated inside cells,
- and the signal is very strong, so there were almost no background
- Even though there were some of debris, they could be come from fixing step.
- No RCA signal were in -RT sample
20x, +RT vs. -RT (1 wash)
Raw images : +RT, -RT |
File:20x 1wash.png |
20x, +RT, NA=0.4 vs. 0.8 (2 wash)
Raw images : NA=0.4, NA=0.8 |
File:20x 2wash 0.4NA 0.8NA.png |
40x, +RT, NA=0.8, Cy3 vs. GFP channel (2 wash)
Raw images : Cy3, GFP |
File:40x 2wash 0.8NA Cy3 GFP.png |
63x, +RT, NA=1.2, Cy3 (2 wash)
Raw images : 63x |
File:63x 1.2NA 2wash 50msec 100EMgain ch00.png |