Hosuk:LabNotes/2013-6-12: Difference between revisions
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*[[Hosuk:Lab_Notes|LabNote]] | |||
====Day 3==== | ====Day 3==== | ||
*Jay finished last step, washing RCA and binding Cy3 probe, and image | *Jay finished last step, washing RCA and binding Cy3 probe, and image | ||
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|Raw images : [[Media:63x_2wash_1.2NA_Cy3.tif|63x]] | |Raw images : [[Media:63x_2wash_1.2NA_Cy3.tif|63x]] | ||
|- | |- | ||
|[[File:63x_1.2NA_2wash_50msec_100EMgain_ch00.png| | |[[File:63x_1.2NA_2wash_50msec_100EMgain_ch00.png|250px]] | ||
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Latest revision as of 17:19, 21 June 2013
Day 3[edit]
- Jay finished last step, washing RCA and binding Cy3 probe, and image
- Rolonies are pretty well generated inside cells,
- and the signal is very strong, so there were almost no background
- Even though there were some of debris, they could be come from fixing step.
- No RCA signal were in -RT sample
20x, +RT vs. -RT (1 wash)[edit]
Raw images : +RT, -RT |
File:20x 1wash.png |
20x, +RT, NA=0.4 vs. 0.8 (2 wash)[edit]
Raw images : NA=0.4, NA=0.8 |
File:20x 2wash 0.4NA 0.8NA.png |
40x, +RT, NA=0.8, Cy3 vs. GFP channel (2 wash)[edit]
Raw images : Cy3, GFP |
File:40x 2wash 0.8NA Cy3 GFP.png |
63x, +RT, NA=1.2, Cy3 (2 wash)[edit]
Raw images : 63x |
File:63x 1.2NA 2wash 50msec 100EMgain ch00.png |