Hosuk:LabNotes/2013-6-12: Difference between revisions

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*[[Hosuk:Lab_Notes|LabNote]]
====Day 3====
====Day 3====
*Jay finished last step, washing RCA and binding Cy3 probe, and image
*Jay finished last step, washing RCA and binding Cy3 probe, and image

Latest revision as of 17:19, 21 June 2013

Day 3[edit]

  • Jay finished last step, washing RCA and binding Cy3 probe, and image
  • Rolonies are pretty well generated inside cells,
  • and the signal is very strong, so there were almost no background
  • Even though there were some of debris, they could be come from fixing step.
  • No RCA signal were in -RT sample


20x, +RT vs. -RT (1 wash)[edit]
Raw images : +RT, -RT
File:20x 1wash.png


20x, +RT, NA=0.4 vs. 0.8 (2 wash)[edit]
Raw images : NA=0.4, NA=0.8
File:20x 2wash 0.4NA 0.8NA.png


40x, +RT, NA=0.8, Cy3 vs. GFP channel (2 wash)[edit]
Raw images : Cy3, GFP
File:40x 2wash 0.8NA Cy3 GFP.png


63x, +RT, NA=1.2, Cy3 (2 wash)[edit]
Raw images : 63x
File:63x 1.2NA 2wash 50msec 100EMgain ch00.png