Matt:LabNotes/2013-6-12: Difference between revisions

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>Mzcai
(Created page with "===Verify Probe Capture=== Primers: <br> CP-2-FA - 'GCACGATCCGACGGTAGTGT'<br> CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'<br> Program 98C 30sec--> (98C 10sec --> 52C 30sec --> 7...")
 
>Mzcai
mNo edit summary
Line 5: Line 5:
CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'<br>
CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'<br>


 
{| {{table}} border = 1
| align="center" style="background:#f0f0f0;"|'''Components'''
| align="center" style="background:#f0f0f0;"|'''Volume'''
|-
| Circle Oligos||x
|-
| 100uM||0.4
|-
| 100uM||0.4
|-
| 2X KAPA SYBG||50
|-
| H2O||x
|-
| Total||100
|-
|
|}




   Program
   Program
   98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> (98C 10sec --> 60C 30sec --> 72C 30sec)x15 cycles--> 72C 3 min --> 15C hold
   98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> (98C 10sec --> 60C 30sec --> 72C 30sec)x15 cycles--> 72C 3 min --> 15C hold

Revision as of 00:21, 13 June 2013

Verify Probe Capture

Primers:
CP-2-FA - 'GCACGATCCGACGGTAGTGT'
CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'

Components Volume
Circle Oligos x
100uM 0.4
100uM 0.4
2X KAPA SYBG 50
H2O x
Total 100


 Program
 98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> (98C 10sec --> 60C 30sec --> 72C 30sec)x15 cycles--> 72C 3 min --> 15C hold