Matt:LabNotes/2013-6-12: Difference between revisions
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>Mzcai (Created page with "===Verify Probe Capture=== Primers: <br> CP-2-FA - 'GCACGATCCGACGGTAGTGT'<br> CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'<br> Program 98C 30sec--> (98C 10sec --> 52C 30sec --> 7...") |
>Mzcai mNo edit summary |
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CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'<br> | CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'<br> | ||
{| {{table}} border = 1 | |||
| align="center" style="background:#f0f0f0;"|'''Components''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume''' | |||
|- | |||
| Circle Oligos||x | |||
|- | |||
| 100uM||0.4 | |||
|- | |||
| 100uM||0.4 | |||
|- | |||
| 2X KAPA SYBG||50 | |||
|- | |||
| H2O||x | |||
|- | |||
| Total||100 | |||
|- | |||
| | |||
|} | |||
Program | Program | ||
98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> (98C 10sec --> 60C 30sec --> 72C 30sec)x15 cycles--> 72C 3 min --> 15C hold | 98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> (98C 10sec --> 60C 30sec --> 72C 30sec)x15 cycles--> 72C 3 min --> 15C hold |
Revision as of 00:21, 13 June 2013
Verify Probe Capture
Primers:
CP-2-FA - 'GCACGATCCGACGGTAGTGT'
CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'
Components | Volume |
Circle Oligos | x |
100uM | 0.4 |
100uM | 0.4 |
2X KAPA SYBG | 50 |
H2O | x |
Total | 100 |
Program 98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> (98C 10sec --> 60C 30sec --> 72C 30sec)x15 cycles--> 72C 3 min --> 15C hold