Matt:LabNotes/2013-6-12: Difference between revisions

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>Mzcai
mNo edit summary
>Mzcai
mNo edit summary
Line 11: Line 11:
| Circle Oligos||x
| Circle Oligos||x
|-
|-
| 100uM||0.4
| 10uM CP-2-FA||1
|-
|-
| 100uM||0.4
| 10uM CP-2-RA||1
|-
|-
| 2X KAPA SYBG||50
| 2X KAPA SYBG||25
|-
|-
| H2O||x
| H2O||x
|-
|-
| Total||100
| Total||50
|-
|-
|  
|  
|}
|}


   Program
   Program
   98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> (98C 10sec --> 60C 30sec --> 72C 30sec)x15 cycles--> 72C 3 min --> 15C hold
   98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> 72C 3 min --> 15C hold

Revision as of 20:43, 13 June 2013

Verify Probe Capture

Primers:
CP-2-FA - 'GCACGATCCGACGGTAGTGT'
CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'

Components Volume
Circle Oligos x
10uM CP-2-FA 1
10uM CP-2-RA 1
2X KAPA SYBG 25
H2O x
Total 50
 Program
 98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> 72C 3 min --> 15C hold