Matt:LabNotes/2013-6-12: Difference between revisions
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>Mzcai mNo edit summary |
>Mzcai mNo edit summary |
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Line 11: | Line 11: | ||
| Circle Oligos||x | | Circle Oligos||x | ||
|- | |- | ||
| | | 10uM CP-2-FA||1 | ||
|- | |- | ||
| | | 10uM CP-2-RA||1 | ||
|- | |- | ||
| 2X KAPA SYBG|| | | 2X KAPA SYBG||25 | ||
|- | |- | ||
| H2O||x | | H2O||x | ||
|- | |- | ||
| Total|| | | Total||50 | ||
|- | |- | ||
| | | | ||
|} | |} | ||
Program | Program | ||
98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 | 98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> 72C 3 min --> 15C hold |
Revision as of 20:43, 13 June 2013
Verify Probe Capture
Primers:
CP-2-FA - 'GCACGATCCGACGGTAGTGT'
CP-2-RA - 'CCGTAATCGGGAAGCTGAAG'
Components | Volume |
Circle Oligos | x |
10uM CP-2-FA | 1 |
10uM CP-2-RA | 1 |
2X KAPA SYBG | 25 |
H2O | x |
Total | 50 |
Program 98C 30sec--> (98C 10sec --> 52C 30sec --> 72C 30sec)x8 cycles --> 72C 3 min --> 15C hold