Hosuk:LabNotesMDA/2013-6-21: Difference between revisions

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===MDA with PGP1F, 4th===
===MDA with PGP1F, 4th===
*MDA run for 8hr
*MDA run [[Hosuk:LabNotesMDA/2013-6-19|@06/19]]
*133mM ALS
*qPCR result was not correct because of using wrong primers --> re-run
*Mixing pump --> shorter mixing time: 1sec period, 5min
**Much less residue were seen under the valve area
 
 
*Push ring mixer volume with air
**Left 5~6 chamber were empty… why so many?
 
*Verification of the amount of amplicon by qPCR : will be done soon
*CoRE fragmentation was done @06/19

Revision as of 17:41, 21 June 2013

MDA with PGP1F, 4th

  • MDA run @06/19
  • qPCR result was not correct because of using wrong primers --> re-run