Hosuk:LabNotesMDA/2013-6-21: Difference between revisions

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*MDA run [[Hosuk:LabNotesMDA/2013-6-19|@06/19]]
*MDA run [[Hosuk:LabNotesMDA/2013-6-19|@06/19]]
*qPCR result was not correct because of using wrong primers --> re-run
*qPCR result was not correct because of using wrong primers --> re-run
**For the initial test, 7 samples - 2, 5, 8, 11, 13, 18, 20, 22 were run.
=====Samples=====
{| {{table}}
|Chamber #
||
|Volume (uL)
||
|Mass. Est. (pg/uL)
||
|Total (pg)
|-
|1
||5.05
|
||
|
||
|-
|2
||5.42
|
||
|
||
|-
|3
||5.36
|
||
|
||
|-
|4
||5.08
|
||
|
||
|-
|5
||6.87
|
||
|
||
|-
|6
||5.46
|
||
|
||
|-
|7
||5.42
|
||
|
||
|-
|8
||5.24
|
||
|
||
|-
|9
||5.22
|
||
|
||
|-
|10
||4.80
|
||
|
||
|-
|11
||5.34
|
||
|
||
|-
|12
||5.15
|
||
|
||
|-
|13
||4.25
|
||
|
||
|-
|14
||5.01
|
||
|
||
|-
|15
||4.83
|
||
|
||
|-
|16
||4.58
|
||
|
||
|-
|17
||5.93
|
||
|
||
|-
|18
||5.74
|
||
|
||
|-
|19
||5.19
|
||
|
||
|-
|20
||5.40
|
||
|
||
|-
|21
||6.02
|
||
|
||
|-
|22
||4.93
|
||
|
||
|-
|23
||5.43
|
||
|
||
|-
|24
||4.60
|
||
|
||
|
|}

Revision as of 17:54, 21 June 2013

MDA with PGP1F, 4th - qPCR

  • MDA run @06/19
  • qPCR result was not correct because of using wrong primers --> re-run
    • For the initial test, 7 samples - 2, 5, 8, 11, 13, 18, 20, 22 were run.


Samples
Chamber # Volume (uL) Mass. Est. (pg/uL) Total (pg)
1 5.05
2 5.42
3 5.36
4 5.08
5 6.87
6 5.46
7 5.42
8 5.24
9 5.22
10 4.80
11 5.34
12 5.15
13 4.25
14 5.01
15 4.83
16 4.58
17 5.93
18 5.74
19 5.19
20 5.40
21 6.02
22 4.93
23 5.43
24 4.60