Hosuk:LabNotesMDA/2013-6-21: Difference between revisions

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**Experimental error, or  
**Experimental error, or  
**Re-hybridization while Neutralization?
**Re-hybridization while Neutralization?
**Higher Tween 20 in master mix?





Revision as of 18:24, 21 June 2013

MDA with PGP1F, 4th - qPCR

  • MDA run @06/19
  • qPCR result was not correct because of using wrong primers --> re-run
    • For the initial test, 7 samples - 2, 5, 11, 13, 18, 20, 22 were run.
10ng 10ng 1ng 1ng 100pg 100pg 10pg 10pg
NTC #2 #5 #11 #13 #18 #20 #22


Result
  • Ladders were correct, but samples were not shown any PCR amplification.
  • MDA didn't work.
  • Possible reason would be
    • Experimental error, or
    • Re-hybridization while Neutralization?


Samples
Chamber # Volume (uL) Mass (pg/uL) Total (pg)
1 5.05
2 5.42
3 5.36
4 5.08
5 6.87
6 5.46
7 5.42
8 5.24
9 5.22
10 4.80
11 5.34
12 5.15
13 4.25
14 5.01
15 4.83
16 4.58
17 5.93
18 5.74
19 5.19
20 5.40
21 6.02
22 4.93
23 5.43
24 4.60