Hosuk:LabNotesMDA/2013-6-21: Difference between revisions
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====Result --> Fail!==== | |||
*Ladders were correct, but samples were not shown any PCR amplification. | *Ladders were correct, but samples were not shown any PCR amplification. | ||
*MDA didn't work. | *MDA didn't work. |
Revision as of 18:27, 21 June 2013
MDA with PGP1F, 4th - qPCR
- MDA run @06/19
- qPCR result was not correct because of using wrong primers --> re-run
- For the initial test, 7 samples - 2, 5, 11, 13, 18, 20, 22 were run.
10ng | 10ng | 1ng | 1ng | 100pg | 100pg | 10pg | 10pg | |||||||
NTC | #2 | #5 | #11 | #13 | #18 | #20 | #22 | |||||||
Result --> Fail!
- Ladders were correct, but samples were not shown any PCR amplification.
- MDA didn't work.
- Possible reason would be
- Experimental error, or
- Re-hybridization while Neutralization?
Samples
Chamber # | Volume (uL) | Mass (pg/uL) | Total (pg) | ||||
1 | 5.05 | ||||||
2 | 5.42 | ||||||
3 | 5.36 | ||||||
4 | 5.08 | ||||||
5 | 6.87 | ||||||
6 | 5.46 | ||||||
7 | 5.42 | ||||||
8 | 5.24 | ||||||
9 | 5.22 | ||||||
10 | 4.80 | ||||||
11 | 5.34 | ||||||
12 | 5.15 | ||||||
13 | 4.25 | ||||||
14 | 5.01 | ||||||
15 | 4.83 | ||||||
16 | 4.58 | ||||||
17 | 5.93 | ||||||
18 | 5.74 | ||||||
19 | 5.19 | ||||||
20 | 5.40 | ||||||
21 | 6.02 | ||||||
22 | 4.93 | ||||||
23 | 5.43 | ||||||
24 | 4.60 | ||||||