Hosuk:LabNotesMDA/2013-6-21: Difference between revisions
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*MDA run [[Hosuk:LabNotesMDA/2013-6-19|@06/19]] | *MDA run [[Hosuk:LabNotesMDA/2013-6-19|@06/19]] | ||
*qPCR result was not correct because of using wrong primers --> re-run | *qPCR result was not correct because of using wrong primers --> re-run | ||
**For the initial test, 7 samples - 2, 5, 11, 13, 18, 20, 22 were run. | |||
{| {{table}} | |||
| align="center" |10ng | |||
|| | |||
| align="center" |10ng | |||
| | |||
| align="center" |1ng | |||
|| | |||
| align="center" |1ng | |||
| | |||
| align="center" |100pg | |||
|| | |||
| align="center" |100pg | |||
| | |||
| align="center" |10pg | |||
|| | |||
| align="center" |10pg | |||
|- | |||
| align="center" |NTC | |||
|| | |||
| align="center" |#2 | |||
| | |||
| align="center" |#5 | |||
|| | |||
| align="center" |#11 | |||
| | |||
| align="center" |#13 | |||
|| | |||
| align="center" |#18 | |||
| | |||
| align="center" |#20 | |||
|| | |||
| align="center" |#22 | |||
|- | |||
| | |||
|} | |||
====Result --> Fail!==== | |||
*Ladders were correct, but samples were not shown any PCR amplification. | |||
*MDA didn't work. | |||
*Possible reason would be | |||
**Experimental error, or | |||
**Re-hybridization while Neutralization? | |||
*Run one more time with the same condition but keeping 1 min. incubation carefully while neutralization, (not too long) |
Latest revision as of 18:30, 21 June 2013
MDA with PGP1F, 4th - qPCR[edit]
- MDA run @06/19
- qPCR result was not correct because of using wrong primers --> re-run
- For the initial test, 7 samples - 2, 5, 11, 13, 18, 20, 22 were run.
10ng | 10ng | 1ng | 1ng | 100pg | 100pg | 10pg | 10pg | |||||||
NTC | #2 | #5 | #11 | #13 | #18 | #20 | #22 | |||||||
Result --> Fail![edit]
- Ladders were correct, but samples were not shown any PCR amplification.
- MDA didn't work.
- Possible reason would be
- Experimental error, or
- Re-hybridization while Neutralization?
- Run one more time with the same condition but keeping 1 min. incubation carefully while neutralization, (not too long)