Hosuk:LabNotesMDA/2013-6-21: Difference between revisions
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**Experimental error, or | **Experimental error, or | ||
**Re-hybridization while Neutralization? | **Re-hybridization while Neutralization? | ||
*Run one more time with the same condition but keeping 1 min. incubation carefully while neutralization, (not too long) | |||
Latest revision as of 18:30, 21 June 2013
MDA with PGP1F, 4th - qPCR[edit]
- MDA run @06/19
- qPCR result was not correct because of using wrong primers --> re-run
- For the initial test, 7 samples - 2, 5, 11, 13, 18, 20, 22 were run.
10ng | 10ng | 1ng | 1ng | 100pg | 100pg | 10pg | 10pg | |||||||
NTC | #2 | #5 | #11 | #13 | #18 | #20 | #22 | |||||||
Result --> Fail![edit]
- Ladders were correct, but samples were not shown any PCR amplification.
- MDA didn't work.
- Possible reason would be
- Experimental error, or
- Re-hybridization while Neutralization?
- Run one more time with the same condition but keeping 1 min. incubation carefully while neutralization, (not too long)