Hosuk:LabNotesMDA/2013-6-21: Difference between revisions

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Line 48: Line 48:
**Experimental error, or  
**Experimental error, or  
**Re-hybridization while Neutralization?
**Re-hybridization while Neutralization?
 
*Run one more time with the same condition but keeping 1 min. incubation carefully while neutralization, (not too long)
 
 
=====Samples=====
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Chamber #'''
||
| align="center" style="background:#f0f0f0;"|'''Volume (uL)'''
||
| align="center" style="background:#f0f0f0;"|'''Mass (pg/uL)'''
||
| align="center" style="background:#f0f0f0;"|'''Total (pg)'''
||
|-
|1
||
|align="center" |5.05
||
|
||
|
|-
|2
||
|align="center" |5.42
||
|
||
|
|-
|3
||
|align="center" |5.36
||
|
||
|
|-
|4
||
|align="center" |5.08
||
|
||
|
|-
|5
||
|align="center" |6.87
||
|
||
|
|-
|6
||
|align="center" |5.46
||
|
||
|
|-
|7
||
|align="center" |5.42
||
|
||
|
|-
|8
||
|align="center" |5.24
||
|
||
|
|-
|9
||
|align="center" |5.22
||
|
||
|
|-
|10
||
|align="center" |4.80
||
|
||
|
|-
|11
||
|align="center" |5.34
||
|
||
|
|-
|12
||
|align="center" |5.15
||
|
||
|
|-
|13
||
|align="center" |4.25
||
|
||
|
|-
|14
||
|align="center" |5.01
||
|
||
|
|-
|15
||
|align="center" |4.83
|
||
|
||
|
|-
|16
||
|align="center" |4.58
||
|
||
|
|-
|17
||
|align="center" |5.93
||
|
||
|
|-
|18
||
|align="center" |5.74
||
|
||
|
|-
|19
||
|align="center" |5.19
||
|
||
|
|-
|20
||
|align="center" |5.40
||
|
||
|
|-
|21
||
|align="center" |6.02
||
|
||
|
|-
|22
||
|align="center" |4.93
||
|
||
|
|-
|23
||
|align="center" |5.43
||
|
||
|
|-
|24
||
|align="center" |4.60
||
|
||
|
|-
|
|}

Latest revision as of 18:30, 21 June 2013

MDA with PGP1F, 4th - qPCR[edit]

  • MDA run @06/19
  • qPCR result was not correct because of using wrong primers --> re-run
    • For the initial test, 7 samples - 2, 5, 11, 13, 18, 20, 22 were run.
10ng 10ng 1ng 1ng 100pg 100pg 10pg 10pg
NTC #2 #5 #11 #13 #18 #20 #22


Result --> Fail![edit]

  • Ladders were correct, but samples were not shown any PCR amplification.
  • MDA didn't work.
  • Possible reason would be
    • Experimental error, or
    • Re-hybridization while Neutralization?
  • Run one more time with the same condition but keeping 1 min. incubation carefully while neutralization, (not too long)