Hosuk:LabNotesMDA/2013-6-19: Difference between revisions

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>Hosuki78
(Created page with "*LabNote ===MDA with PGP1F, 4th=== *MDA run for 8hr @06/19 *133mM ALS *Mixing pump --> shorter mixing time: 1sec period, 5min **Much less residue were...")
 
>Hosuki78
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Line 13: Line 13:
*Verification of the amount of amplicon by qPCR : will be done soon
*Verification of the amount of amplicon by qPCR : will be done soon
*CoRE fragmentation was done @06/19
*CoRE fragmentation was done @06/19
=====Samples=====
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Chamber #'''
||
| align="center" style="background:#f0f0f0;"|'''Volume (uL)'''
||
| align="center" style="background:#f0f0f0;"|'''Mass (pg/uL)'''
||
| align="center" style="background:#f0f0f0;"|'''Total (pg)'''
||
|-
|1
||
|align="center" |5.05
||
|
||
|
|-
|2
||
|align="center" |5.42
||
|
||
|
|-
|3
||
|align="center" |5.36
||
|
||
|
|-
|4
||
|align="center" |5.08
||
|
||
|
|-
|5
||
|align="center" |6.87
||
|
||
|
|-
|6
||
|align="center" |5.46
||
|
||
|
|-
|7
||
|align="center" |5.42
||
|
||
|
|-
|8
||
|align="center" |5.24
||
|
||
|
|-
|9
||
|align="center" |5.22
||
|
||
|
|-
|10
||
|align="center" |4.80
||
|
||
|
|-
|11
||
|align="center" |5.34
||
|
||
|
|-
|12
||
|align="center" |5.15
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|
||
|
|-
|13
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|align="center" |4.25
||
|
||
|
|-
|14
||
|align="center" |5.01
||
|
||
|
|-
|15
||
|align="center" |4.83
|
||
|
||
|
|-
|16
||
|align="center" |4.58
||
|
||
|
|-
|17
||
|align="center" |5.93
||
|
||
|
|-
|18
||
|align="center" |5.74
||
|
||
|
|-
|19
||
|align="center" |5.19
||
|
||
|
|-
|20
||
|align="center" |5.40
||
|
||
|
|-
|21
||
|align="center" |6.02
||
|
||
|
|-
|22
||
|align="center" |4.93
||
|
||
|
|-
|23
||
|align="center" |5.43
||
|
||
|
|-
|24
||
|align="center" |4.60
||
|
||
|
|-
|
|}

Latest revision as of 18:30, 21 June 2013

MDA with PGP1F, 4th[edit]

  • MDA run for 8hr @06/19
  • 133mM ALS
  • Mixing pump --> shorter mixing time: 1sec period, 5min
    • Much less residue were seen under the valve area


  • Push ring mixer volume with air
    • Left 5~6 chamber were empty… why so many?
  • Verification of the amount of amplicon by qPCR : will be done soon
  • CoRE fragmentation was done @06/19


Samples[edit]
Chamber # Volume (uL) Mass (pg/uL) Total (pg)
1 5.05
2 5.42
3 5.36
4 5.08
5 6.87
6 5.46
7 5.42
8 5.24
9 5.22
10 4.80
11 5.34
12 5.15
13 4.25
14 5.01
15 4.83
16 4.58
17 5.93
18 5.74
19 5.19
20 5.40
21 6.02
22 4.93
23 5.43
24 4.60